Evidence map›Paper›PMID 39966254›Full record

ArticleMikrochimica acta2025

Lateral flow biosensor development for the visual identification of H1N1 virus based on primer extension nucleic acid isothermal amplification and M13mp18 single-stranded DNA.

Lingling Wang, Shengjun Bu, Heyuan Meng, Xiaoying Zhang, Hongyu Zhou, Chuanna Xu, Zhuo Hao, Yuchun Su, Jiayu Wan

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lingling WangCollege of Life Sciences, Jilin Agricultural University, Changchun, 130118, China.
Shengjun BuChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China.
Heyuan MengChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China.
Xiaoying ZhangCollege of Life Sciences, Jilin Agricultural University, Changchun, 130118, China.
Hongyu ZhouChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China.
Chuanna XuSchool of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
Zhuo HaoChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China.
Yuchun SuCollege of Life Sciences, Jilin Agricultural University, Changchun, 130118, China. suyuchun@126.com.
Jiayu WanChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China. wanjiayu@hotmail.com.

Funding

Science and Technology Development Plans of Jilin Province 20230203143SF, 20240101203JC
6 · The paper itself

Abstract

A lateral flow biosensor was developed based on newly established primer extension nucleic acid isothermal amplification (PEIA) and M13mp18 single-stranded DNA (ssDNA) to visually identify the H1N1 virus. The PEIA process was primer based on a template to continuously extend multiple repeat sequences, using DNA polymerase, to form long ssDNAs. M13mp18 ssDNA was then coupled to multiple long ssDNAs from PEIA and functionalized in a signal tag DNA nanostructure (STDN) for the lateral flow biosensor. In the presence of target H1N1-RNA, the sandwich structure capture probe/H1N1-RNA/STDN is fabricated caught in the lateral flow biosensor test zone, and appears red. Our biosensor successfully identified H1N1 virus RNA over a broad range of concentrations (10-10

Indexed as

Biosensing TechniquesDNA, Single-StrandedInfluenza A Virus, H1N1 SubtypeNucleic Acid Amplification TechniquesDNA PrimersHumansLimit of DetectionRNA, ViralDNA PrimersDNA, Single-StrandedRNA, ViralInfluenza virusIsothermal amplificationLateral flow assayM13mp18 ssDNA

Identifiers

PMID39966254

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.