Evidence map›Paper›PMID 39966227›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2025

BMAL1 Regulates Collagen Production in the Myometrium and Leiomyomas.

Yidan Dai, Masanori Ono, Tomoo Suzuki, Shigehiro Hayashi, Junya Kojima, Toru Sasaki, Tomoko Fujiwara, Takiko Daikoku, Jumpei Terakawa, Yoshiko Maida and 4 more

Abstract read
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In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yidan DaiDepartment of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, 160-0023, Japan.
Masanori OnoDepartment of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, 160-0023, Japan. masanori@tokyo-med.ac.jp.ORCID 0000-0001-9249-6813
Tomoo SuzukiDepartment of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, 160-0023, Japan.
Shigehiro HayashiDepartment of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, 160-0023, Japan.
Junya KojimaDepartment of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, 160-0023, Japan.
Toru SasakiDepartment of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, 160-0023, Japan.
Tomoko FujiwaraDepartment of Human Life Environments, Kyoto Notre Dame University, Kyoto, 606-0848, Japan.
Takiko DaikokuDivision of Animal Disease Model, Research Center for Experimental Modeling of Human Disease, Graduate School of Medical Science, Kanazawa University, Kanazawa, 920-8641, Japan.
Jumpei TerakawaSchool of Veterinary Medicine, Azabu University, Kanagawa, 252-0206, Japan.
Yoshiko MaidaDepartment of Nursing, College of Medical, Pharmaceutical, and Health Sciences, Kanazawa University, Kanazawa, 920-8641, Japan.
Hitoshi AndoDepartment of Cellular and Molecular Function Analysis, Graduate School of Medical Science, Kanazawa University, Kanazawa, 920-8641, Japan.
Hiroshi FujiwaraDepartment of Obstetrics and Gynecology, Graduate School of Medical Science, Kanazawa University, Kanazawa, 920-8641, Japan.
Naoaki KujiDepartment of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, 160-0023, Japan.
Hirotaka NishiDepartment of Obstetrics and Gynecology, Tokyo Medical University, Tokyo, 160-0023, Japan.

Funding

JSPS JP20H03996JSPS JP21H04837JSPS JP21K09512JSPS JP21K18297JSPS JP22K09556
6 · The paper itself

Abstract

Infertility and reproductive issues are commonly observed in animals with clock abnormalities. Substantial rodent data is available; however, relatively few studies have investigated the connection between fertility and clock abnormalities in humans. Therefore, this study aimed to analyze the expression of circadian clock genes and their impact on genes involved in collagen production in the human myometrium and leiomyomas (LM). The relationship between the expression of brain and muscle aryl-hydrocarbon-receptor-nuclear-translocator (Arnt)-like protein-1 (BMAL1) and the genes responsible for collagen synthesis in the human MM and LMs were investigated. Human MM and LM tissues were collected for analysis from patients who underwent hysterectomy analysis. Immunohistochemical analysis, cell culturing, immunofluorescence, small interfering RNA transfection, reverse transcription quantitative real-time polymerase chain reaction, scratch wound assays, and transwell assays were employed to gain a comprehensive understanding of the cellular and molecular processes. A correlation was found between BMAL1 expression and genes regulating collagen synthesis in primary cultures of human MM and LM cells. Moreover, the inhibition of BMAL1 differentially increased the migration and invasion of MM and LM cells. This work discloses the role of BMAL1 in collagen production in primary cultures of human MM and LM cells, offering insight into clock gene involvement in both normal and pathological uterine conditions. Furthermore, this study highlights the crucial role of BMAL1 in collagen synthesis in human MM and LM cells, underscoring the significance of BMAL1 in the regulation of reproductive physiology. These results suggest that BMAL1 might be a useful target molecule for anti-LM therapy.

Indexed as

ARNTL Transcription FactorsCollagenLeiomyomaMyometriumUterine NeoplasmsAdultCell MovementFemaleHumansMiddle AgedARNTL Transcription FactorsBMAL1 protein, humanCollagenBMAL1Circadian clockCollagen synthesisLeiomyomaMyometrium

Identifiers

PMID39966227

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.