Evidence map›Paper›PMID 39966174›Full record

ArticleAnalytical and bioanalytical chemistry2025

Cost-effective in-house-made whole blood materials for internal quality control in clinical flow cytometry analysis.

Hui-Min Chong, Zhao-Wei Zhang, Jin-Mi Li, Xiao-Dong Ren, Chun-Mei Gong, Zhi-Xian Zhu, Nan Xiang, Zhong-Hua Ni, Qing Huang

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hui-Min ChongDepartment of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing, 400042, China.ORCID http://orcid.org/0000-0001-8335-7352
Zhao-Wei ZhangDepartment of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing, 400042, China.ORCID http://orcid.org/0009-0007-3007-7374
Jin-Mi LiDepartment of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing, 400042, China.ORCID http://orcid.org/0000-0003-1507-3889
Xiao-Dong RenDepartment of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing, 400042, China.ORCID http://orcid.org/0000-0003-1172-5579
Chun-Mei GongDepartment of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing, 400042, China.
Zhi-Xian ZhuSchool of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China.
Nan XiangSchool of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China.
Zhong-Hua NiSchool of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, 211189, China.
Qing HuangDepartment of Laboratory Medicine, Daping Hospital, Army Medical University, Chongqing, 400042, China. qinghuang@tmmu.edu.cn.ORCID http://orcid.org/0000-0002-0140-8978

Funding

Clinical Medical Innovation Capacity Cultivation Program of the Army Characteristic Medical Center ZXZYTSLC11Key Program of Technology Innovation and Application Development of Chongqing CSTB2022TIAD-KPX0190National Natural Science Foundation of China Grant No. 82172370
6 · The paper itself

Abstract

Internal quality control (IQC) is essential for ensuring the accuracy of results in clinical trials. However, there is a significant shortage of commercial quality control materials for IQC in flow cytometry. This study aimed to develop cost-effective in-house-made whole blood materials from clinically discarded samples to serve as IQC in clinical flow cytometry analysis. Discarded clinical whole blood samples were collected to prepare red blood cell (RBC) suspensions through density centrifugation. White blood cell (WBC) suspensions were prepared using made in-house (MiH) lysing buffer, followed by fixation with the MiH fixative solution. The in-house-made whole blood materials were then prepared by mixing the RBC and WBC suspensions. These mixtures were stored under controlled temperature conditions to ensure long-term stability. These materials are intended for use in internal quality control (IQC) for clinical flow cytometry analysis. The recovery rate of RBC suspensions achieved through density centrifugation was 95%. Different blood type RBC suspensions were effectively preserved in Alsever's solution via plasma washing and re-mixing. The average viability of MiH WBC suspensions was 97%, with a recovery rate of 84%, both significantly higher than those observed with ACK (p < 0.001). Among the in-house-made whole blood material samples, sample a1-which included plasma, Alsever's solution, RBC suspension, WBC suspension, and Proclin 300-exhibited the best stability in flow cytometry, demonstrating stable expression of cell antigens for over 5 months. The in-house-made whole blood materials proved to be cost-effective and suitable for use in IQC for clinical flow cytometry analysis.

Indexed as

Flow CytometryCost-Benefit AnalysisErythrocytesHumansLeukocytesQuality ControlCell fixationCell suspensionsDiscarded clinical whole bloodFlow cytometryIn-house-made

Identifiers

PMID39966174

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.