Evidence map›Paper›PMID 39964910›Full record

ArticleElectrophoresis2025

Design Guidelines of Free-Flow Counterflow Gradient Focusing Device for Protein Fractionation.

Matthew Courtney, Oscar Manuel Carreno-Molina, Carolyn L Ren

Abstract read
In one paragraph

Article in Electrophoresis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Matthew CourtneyDepartment of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, Ontario, Canada.
Oscar Manuel Carreno-MolinaDepartment of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, Ontario, Canada.
Carolyn L RenDepartment of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, Ontario, Canada.

Funding

Canada Research Chairs CRC-2021-00057Natural Sciences and Engineering Research Council of Canada (NSERC) RGPIN-04151-2018
6 · The paper itself

Abstract

Free-flow counterflow gradient focusing (FF-CGF) is a promising tool for protein fractionation. This work establishes guidelines to construct an FF-CGF system suitable for preparative applications. Numerical and analytical computations are undertaken to investigate design parameters, including device geometry, flow conditions, sample concentration, and applied voltage. This collective information serves as a design tool to guide the fabrication of the device, which, in this case, is constructed from polymethyl methacrylate (PMMA) and double-sided tape. Furthermore, the design tool also supports device operation and provides insights into the expected resolution and throughput. Experimental results, which demonstrated the ability of the designed chip to generate a uniform counterflow gradient while separating small molecules and proteins, validated the accuracy of the design guidelines.

Indexed as

Microfluidic Analytical TechniquesProteinsEquipment DesignPolymethyl MethacrylatePolymethyl MethacrylateProteinscounterflow gradient focusing | free‐flow electrophoresis | peak dispersion | protein purification

Identifiers

PMID39964910
PMCPMC12449751

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.