Evidence map›Paper›PMID 39964719›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Viral circular RNA-encoded protein, ceVP28, divulges an antiviral response in invertebrates.

Sirawich Limkul, Tannatorn Phiwthong, Supitcha Wanvimonsuk, Tuangrak Seabkongseng, Phirom Aunkam, Phattarunda Jaree, Waruntorn Luangtrakul, Kanjana Mahanil, Kamonluck Teamtisong, Panlada Tittabutr and 5 more

Abstract read
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In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Animal virus-host interactions mediated by non-coding RNAs.Frontiers in cellular and infection microbiology · 2026
    Review
  3. Roles and Applications of Circular RNA in Virus Infection.International journal of molecular sciences · 2025
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Sirawich Limkul *School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Tannatorn Phiwthong *School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Supitcha Wanvimonsuk *Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.ORCID 0000-0001-8115-9272
Tuangrak SeabkongsengSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Phirom AunkamSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Phattarunda JareeCenter of Applied Shrimp Research and Innovation, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Waruntorn LuangtrakulCenter of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Kanjana MahanilSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Kamonluck TeamtisongThe Center for Scientific and Technological Equipment, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Panlada TittabutrSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Neung TeaumroongSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Peter SarnowDepartment of Microbiology and Immunology, School of Medicine, Stanford University, Stanford, CA 94305.ORCID 0000-0002-2043-2770
Han-Ching WangDepartment of Biotechnology and Bioindustry Sciences, College of Biosciences and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.ORCID 0000-0001-7283-4942
Kunlaya SomboonwiwatCenter of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Pakpoom BoonchuenSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.

Funding

Suranaree University of Technology (SUT) N/A
6 · The paper itself

Abstract

Invertebrates mostly use innate immunity to counteract pathogenic infections. In this study, shrimp was used as a model organism to explore the functions of circular RNAs (circRNAs) derived from white spot syndrome virus (WSSV). We identified four viral circRNAs, termed circWSSV147, circWSSV326, circWSSV458, and circVP28, from transcriptomic data of WSSV-infected shrimp. CircVP28, which contains an internal ribosome entry site, was further characterized to determine its potential as a template for protein translation. We observed the presence of a truncated, circRNA-encoded VP28 (ceVP28) in infected shrimp. Both ceVP28 and its parental counterpart, VP28, share the same host cell binding partner Rab7, which is a host receptor for WSSV. Coadministration of recombinant ceVP28 protein and WSSV to penaeid shrimps reduced both viral copy numbers and mortality upon WSSV challenges. These findings uncovered a host defense mechanism by which a protein encoded by a viral circRNA modulates virus-receptor interactions, resulting in blocking of viral entry.

Indexed as

PenaeidaeRNA, CircularRNA, ViralViral ProteinsWhite spot syndrome virus 1AnimalsImmunity, Innaterab7 GTP-Binding Proteinsrab GTP-Binding ProteinsViral Envelope Proteinsrab7 GTP-Binding Proteinsrab GTP-Binding ProteinsRNA, CircularRNA, ViralViral Envelope ProteinsViral ProteinsVP28 protein, white spot syndrome viruscircRNA-encoded proteinshrimpviral circRNAviral entrywhite spot syndrome virus

Identifiers

PMID39964719
PMCPMC11874341

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.