ArticleTropical animal health and production2025
Resveratrol and antifreeze protein type I show no synergistic benefits in cryopreserved ram sperm.
Article in Tropical animal health and production, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Nicotinic and Folic Acid Supplementation Improves Cryopreserved Ram Sperm Quality, Potentially Through Modulation ofInternational journal of molecular sciences · 2026Article
- Liquid Storage and Cryopreservation of Ram Semen: Storage-Associated Damage and the Role of Non-Enzymatic Antioxidants.Veterinary sciences · 2026Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study investigated the effect of resveratrol (RV) after thawing of ram sperm cryopreserved with or without the addition of antifreeze protein I (AFPI) on sperm quality during the 3 h incubation period. Each ram ejaculate was split into two aliquots, diluted in a TRIS egg yolk (TEY) extender supplemented with 0 (control) or 0.1 µg/mL AFPI, and then cooled and frozen. Thawed samples of both treatments were subdivided and incubated in Talp-Hepes medium with 0 (TEY and AFPI group) or 50 µM of RV (TEY + RV group and AFPI + RV group) for 3 h at 38 ºC. The sperm kinetics, capacitation status, plasma membrane integrity, mitochondrial activity, sperm-egg binding assay, and TBARS concentrations were evaluated at different intervals. Data were analyzed by mixed model, including the incubation time, AFP treatment, RV treatment, and their interactions as main effects, and the day of semen collection and the ram as random effects (P < 0.05; LS mean ± pooled SEM). The AFPI, RV treatments, and their interaction did not affect the average total motility (~ 17.1 ± 2.4%), progressive motility (~ 5.6 ± 1.4%), mitochondrial activity (~ 35.5 ± 2.3 arbitrary unit), plasma membrane integrity (~ 18.2 ± 2.4%), sperm binding capacity (~ 2.2 ± 0.1 sperm per mm
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Identifiers
39964625What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.