Evidence map›Paper›PMID 39964595›Full record

ArticleApplied biochemistry and biotechnology2025

Phytochemical-Mediated Green Synthesis of Silver, Copper, and Ag-Cu Bimetallic Nanoparticles Using Peganum harmala Demonstrating Advanced Catalytic, Antioxidant, and Biomedical Applications.

Amjid Khan, Muhammad Anas, Fouzia Bibi, Muhammad Ali, Ali Talha Khalil, Khurram Shahzad Munawar, Hamza Elsayed Ahmed Mohamed, Khaoula Hkiri, Malik Maaza, Zabta Khan Shinwari

Abstract read
PubMed Publisher
In one paragraph

Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. RSC advances · 2026
    Article
  2. Review
  3. Article
  4. Article
  5. Eco-friendly Cu-TiOAMB Express · 2026
    Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Amjid KhanDepartment of Plant Sciences, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan. amjidkhan@bs.qau.edu.pk.ORCID http://orcid.org/0009-0001-5475-5156
Muhammad AnasDepartment of Plant Sciences, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan.
Fouzia BibiDepartment of Plant Sciences, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan.
Muhammad AliDepartment of Biotechnology, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan.
Ali Talha KhalilDepartment of Pathology, Lady Reading Hospital Medical Teaching Institution, Peshawar, 25000, KP, Pakistan.
Khurram Shahzad MunawarDepartment of Chemistry, University of Mianwali, Mianwali, 42200, Punjab, Pakistan.
Hamza Elsayed Ahmed MohamedCollege of Graduate Studies, UNESCO UNISA Africa Chair in Nanoscience and Nanotechnology, University of South Africa, Pretoria, South Africa.
Khaoula HkiriCollege of Graduate Studies, UNESCO UNISA Africa Chair in Nanoscience and Nanotechnology, University of South Africa, Pretoria, South Africa.
Malik MaazaCollege of Graduate Studies, UNESCO UNISA Africa Chair in Nanoscience and Nanotechnology, University of South Africa, Pretoria, South Africa.
Zabta Khan ShinwariDepartment of Plant Sciences, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan. shinwari2008@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study explores the green synthesis of silver (Ag-NPs), copper (Cu-NPs), and silver-copper bimetallic nanoparticles (Ag-Cu Bimetallic NPs) using the leaf extract of Peganum harmala, a sustainable and renewable plant source. This eco-friendly synthesis method avoids hazardous chemicals and minimizes waste, advancing the principles of green nanotechnology. Characterization demonstrated distinct properties; Ag-NPs, Cu-NPs, and Ag-Cu bimetallic NPs exhibited surface plasmon resonance peaks at 425 nm, 555 nm, and 525 nm, respectively, and crystallite sizes of 21.42 nm, 21.40 nm, and 26.29 nm as confirmed by X-ray diffraction. Morphological analysis revealed spherical Ag-NPs, flake-like Cu-NPs, and a mix of cubic and spherical Ag-Cu NPs. Catalytic activity tests showed Ag-Cu bimetallic NPs achieved 95% degradation of methylene blue dye, highlighting their potential in environmental remediation. Antibacterial assays demonstrated that Ag-NPs exhibited the highest inhibition zones against Bacillus subtilis (27.0 mm) and Escherichia coli (27.67 mm), while Cu-NPs were effective against Klebsiella pneumoniae at higher concentrations. Ag-NPs also exhibited significant antifungal activity, particularly against Aspergillus niger (15.00 mm). Antioxidant assays revealed Ag-Cu bimetallic NPs displayed the highest free radical scavenging capacity (59.18%), followed by Ag-NPs (35.41%). Cytotoxicity tests indicated Ag-Cu bimetallic NPs had an IC

Indexed as

AntioxidantsCopperGreen Chemistry TechnologyMetal NanoparticlesPeganumPhytochemicalsSilverAnti-Bacterial AgentsCatalysisHumansMicrobial Sensitivity TestsPlant ExtractsPlant LeavesAnti-Bacterial AgentsAntioxidantsCopperPhytochemicalsPlant ExtractsSilverAntioxidantBiomedical applicationsGreen synthesisNanobiotechnologyPhytochemicals

Identifiers

PMID39964595

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.