ArticleClinical oral investigations2025
Impact of endodontic irrigant solutions on the viability and metabolism of stem cells from apical papilla: proposal of a novel in vitro model.
Article in Clinical oral investigations, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Impact of EDTA Formulations and Activation Methods on Stem Cell Response, Antimicrobial Activity and Dentine Integrity: An In Vitro Study.Australian endodontic journal : the journal of the Australian Society of Endodontology Inc · 2026Article
- Biological, antimicrobial, and apical tissue dissolution effects of ambroxol hydrochloride as an irrigant for regenerative endodontics: an in vitro study.BMC oral health · 2026Article
- A comprehensive evaluation of the antimicrobial efficacy of concentrated growth factor against biofilms associated with endodontic diseases: An in vitro study.Journal of clinical and experimental dentistry · 2026Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
objectivesThis study aimed to evaluate the effect of different irrigation protocols on the viability and metabolism of stem cells from the apical papilla (SCAP) using a new in vitro protocol that simulates the clinical situation. MATERIALS AND
methodsForty-eight bovine dentin cylinders were obtained and prepared to simulate teeth with incomplete rhizogenesis, positioned under a three-dimensional (3D) culture of SCAPs to mimic the apical papilla. The cylinders were divided into four groups (n = 8) according to the irrigating solution: Control; NaOCl (Sodium hypochlorite 1%); EDTA (17% ethylenediaminetetraacetic acid); and NaOCl + EDTA. Subsequently, the viability (Live/Dead n = 2) and metabolism (Alamar Blue n = 6) of the cells were assessed (ISO 10993). Data were analyzed using Kruskal-Wallis and Dunn tests (p < 0.05).
resultsIn the 1 to 3 days period, Control and EDTA had significantly higher increases in metabolism compared to NaOCl and NaOCl + EDTA (p < 0.05). In the 3- to 7-day period, metabolism significantly decreased in NaOCl + EDTA compared to EDTA (p < 0.05) but was similar to Control and NaOCl. Additionally, significant differences were observed within groups Control, EDTA, and NaOCl + EDTA across the two periods (p < 0.05).
conclusionsThe tested in vitro model allows for the analysis of the response of SCAPs to different irrigating solutions, simulating the clinical situation. Sodium hypochlorite 1% demonstrated high cytotoxicity to SCAPs, whose effects were partially reversed by 17% EDTA. CLINICAL RELEVANE: The methodology developed provides a tool for future investigations, allowing for the assessment of new irrigants and techniques that may optimize tissue regeneration.
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Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.