Evidence map›Paper›PMID 39963999›Full record

ArticleClinical and experimental immunology2025

Nck1 regulates the in vitro development of human regulatory T cells through AKT pathway.

Aussanee Nuiyen, Donruedee Sanguansermsri, Jarun Sayasathid, Kanthachat Thatsakorn, Siraphop Thapmongkol, Jatuporn Ngoenkam, Sutatip Pongcharoen

Abstract read
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In one paragraph

Article in Clinical and experimental immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Aussanee NuiyenDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.ORCID 0000-0003-4681-6091
Donruedee SanguansermsriDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.
Jarun SayasathidDivision of Cardiothoracic Surgery, Department of Surgery, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand.
Kanthachat ThatsakornDivision of Cardiothoracic Surgery, Department of Surgery, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand.
Siraphop ThapmongkolDivision of Cardiothoracic Surgery, Department of Surgery, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand.
Jatuporn NgoenkamDepartment of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.
Sutatip PongcharoenDivision of Immunology, Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand.

Funding

Naresuan University N42A650331National Research Council of ThailandNational Science Research and Innovation Fund R2567B020Royal Golden JubileeThailand Science Research and Innovation PHD/0085/2561
6 · The paper itself

Abstract

T cell receptor (TCR) signalling is crucial in determining the fate of thymocyte differentiation in the thymus. The high-avidity interaction between TCR and self-peptide-MHC complexes induces development of regulatory T cells (Tregs), lineage commitment for which is controlled by expression of transcription factor Forkhead box P3 (FoxP3). The non-catalytic region of the tyrosine kinase (Nck) comprises two members, Nck1 and Nck2, with Nck1 playing a dominant role in TCR-mediated T cell activation and function. Nck's role, while established in thymocyte development, remains unelucidated in development of Tregs. In this study, we aimed to determine the function of Nck1 in the in vitro development and differentiation of human thymocytes. Human thymocytes were transfected with shRNA plasmid to silence Nck1 expression. The number of FoxP3+ Tregs decreased noticeably in Nck1 knockdown thymocytes after co-cultivation with myeloid dendritic cells (mDCs) and thymic epithelial cells for 14 days. Furthermore, decreased phosphorylation of AKT and FoxO1 was observed in Nck1-silenced thymocytes, in association with reduced FoxO1 nuclear localization. Taken together, these findings identify the pivotal role of Nck1 in Treg development.

Indexed as

Adaptor Proteins, Signal TransducingOncogene ProteinsProto-Oncogene Proteins c-aktThymocytesT-Lymphocytes, RegulatoryCell DifferentiationCells, CulturedCoculture TechniquesDendritic CellsForkhead Box Protein O1Forkhead Transcription FactorsHumansLymphocyte ActivationPhosphorylationReceptors, Antigen, T-CellRNA, Small InterferingAdaptor Proteins, Signal TransducingForkhead Box Protein O1Forkhead Transcription FactorsFOXO1 protein, humanFOXP3 protein, humanNck proteinOncogene ProteinsProto-Oncogene Proteins c-aktReceptors, Antigen, T-CellRNA, Small InterferingFoxO1FoxP3Nck1PI3K/AKT pathwayregulatory T cellthymocyte development

Identifiers

PMID39963999
PMCPMC11923542

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.