Evidence map›Paper›PMID 39963424›Full record

ArticleComputational and structural biotechnology journal2024

Nanopore sequencing enables novel detection of deuterium incorporation in DNA.

Christian Höner Zu Siederdissen, Jannes Spangenberg, Kevin Bisdorf, Sebastian Krautwurst, Akash Srivastava, Manja Marz, Martin Taubert

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Computational and structural biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Microbial Ecotoxicology-40 Years on.Life (Basel, Switzerland) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Christian Höner Zu SiederdissenRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University Jena, Jena, Germany.
Jannes SpangenbergRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University Jena, Jena, Germany.
Kevin BisdorfAquatic Geomicrobiology, Institute of Biodiversity, Faculty of Biological Sciences, Friedrich Schiller University Jena, Dornburger Str. 159, 07743 Jena, Germany.
Sebastian KrautwurstRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University Jena, Jena, Germany.
Akash SrivastavaRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University Jena, Jena, Germany.
Manja MarzRNA Bioinformatics and High-Throughput Analysis, Friedrich Schiller University Jena, Jena, Germany.
Martin TaubertAquatic Geomicrobiology, Institute of Biodiversity, Faculty of Biological Sciences, Friedrich Schiller University Jena, Dornburger Str. 159, 07743 Jena, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identifying active microbes is crucial to understand their role in ecosystem functions. Metabolic labeling with heavy, non-radioactive isotopes, i.e., stable isotope probing (SIP), can track active microbes by detecting heavy isotope incorporation in biomolecules such as DNA. However, the detection of heavy isotope-labeled nucleotides directly during sequencing has, to date, not been achieved. In this study, Oxford nanopore sequencing was utilized to detect heavy isotopes incorporation in DNA molecules. Two isotopes widely used in SIP experiments were employed to label a bacterial isolate: deuterium (D, as D

Indexed as

13C labelingBayesian logistic regressionD2O labelingDeuteriumOxford nanopore sequencingStable isotope probing

Identifiers

PMID39963424
PMCPMC11832021

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.