Evidence map›Paper›PMID 39963195›Full record

ArticlePeerJ2025

Increased glycoprotein hormone yield in stably transfected CHO cells using human serum albumin signal peptide for beta-chains.

Maria V Sinegubova, Denis E Kolesov, Ivan I Vorobiev, Nadezhda A Orlova

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Maria V SinegubovaLaboratory of Mammalian Cell Bioengineering, Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.ORCID 0000-0002-8950-6061
Denis E KolesovLaboratory of Mammalian Cell Bioengineering, Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.ORCID 0000-0002-9230-3433
Ivan I VorobievLaboratory of Mammalian Cell Bioengineering, Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.ORCID 0000-0003-0064-4458
Nadezhda A OrlovaLaboratory of Mammalian Cell Bioengineering, Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.ORCID 0000-0001-9483-2892

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heterologous signal peptides enable increasing titers of recombinant proteins in mammalian cell culture. Four human heterodimeric glycoprotein hormones (follicle-stimulating hormone, FSH; luteinizing hormone, LH; chorionic gonadotropin, CG; and thyroid-stimulating hormone, TSH) were expressed in stably transfected CHO cells when varying signal peptides of their β-subunits. The signal peptide of human serum albumin proved to be the most effective for the glycoprotein hormone family. The cell specific productivity was increased for LH (2.5 pg/cell, 4-fold increase), TSH (1.6 pg/cell, 13-fold increase), and CG (1.0 pg/cell, 60%-increase). According to the Western blotting and quantitative PCR data, the productivity increase is associated with an increase in the efficiency of translation and translocation of β-subunits of hormones in the endoplasmic reticulum due to their coupling with the heterologous signal peptides.

Indexed as

GlycoproteinsProtein Sorting SignalsSerum Albumin, HumanAnimalsCHO CellsChorionic GonadotropinCricetinaeCricetulusFollicle Stimulating HormoneHumansLuteinizing HormoneRecombinant ProteinsThyrotropinTransfectionChorionic GonadotropinFollicle Stimulating HormoneGlycoproteinsLuteinizing HormoneProtein Sorting SignalsRecombinant ProteinsSerum Albumin, HumanThyrotropinCHO cell cultureGlycoprotein hormonesGonadotropinsHuman chorionic gonadotropin (hCG)Human follicle stimulating hormone (hFSH)Human luteinizing hormone (hLH)Human thyroid stimulating hormone (hTSH)Recombinant protein productionSignal peptide

Identifiers

PMID39963195
PMCPMC11831970

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.