ArticleBMC musculoskeletal disorders2025
Exploring the correlation between magnetic resonance diffusion tensor imaging (DTI) parameters and aquaporin expression and biochemical composition content in degenerative intervertebral disc nucleus pulposus tissue: a clinical experimental study.
Article in BMC musculoskeletal disorders, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- Research progress of diffusion tensor imaging in lumbar degenerative diseases: a narrative review.Journal of orthopaedic surgery and research · 2026Review
- Influence of the Intervertebral Disc Microenvironment on Matrix Synthesis and Metabolism in Goat Nucleus Pulposus Cells.JOR spine · 2026Article
- Integrating cells, scaffolds, and molecular regulation: a mechanobiological and translational review of bioengineering therapies for intervertebral disc degeneration.Frontiers in bioengineering and biotechnology · 2026Review
- Bioengineering Support in the Assessment and Rehabilitation of Low Back Pain.Bioengineering (Basel, Switzerland) · 2025Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
objectiveThis study investigates the correlation between magnetic resonance diffusion tensor imaging (DTI) parameters and biochemical composition in degenerative intervertebral disc nucleus pulposus tissue, offering a potential reference for the clinical diagnosis and efficacy evaluation of intervertebral disc degeneration.
methodsHuman lumbar intervertebral disc nucleus pulposus tissue samples were collected via full endoscopic minimally invasive surgery. DTI was employed to quantitatively measure fractional anisotropy (FA) and apparent diffusion coefficient (ADC) values in the degenerative nucleus pulposus, examining the relationship between Pfirrmann grading and these DTI parameters. Western blotting was used to detect the expression levels of aquaporin 1 (AQP1) and aquaporin 3 (AQP3) in the degenerative tissue. The glycosaminoglycan (GAG) content was quantified using the dimethylmethylene blue (DMMB) colorimetric assay, and collagen content was assessed with the Sircol soluble collagen assay kit. The relationship between Pfirrmann grading and biochemical composition was also analyzed. Finally, correlation analysis was performed between the FA and ADC values from the human nucleus pulposus tissue and their GAG and collagen contents.
resultsA total of 39 patients (19 males, 20 females) with lumbar disc herniation (LDH), averaging 54.41 years of age, were included. As the degree of Pfirrmann degeneration increased, FA values rose, while ADC values continuously declined. Concurrently, as degeneration progressed, expression of AQP1 and AQP3 proteins decreased, GAG content significantly diminished, and collagen content increased. FA values exhibited a moderate negative correlation with GAG content (r = -0.5974, P < 0.0001) and a strong positive correlation with collagen content (r = 0.8694, P < 0.0001). ADC values showed a moderate positive correlation with GAG content (r = 0.6873, P < 0.0001) and a strong negative correlation with collagen content (r = -0.8502, P < 0.0001).
conclusionThe FA and ADC values derived from magnetic resonance DTI, along with the protein expression levels of AQPs, can reflect the severity of intervertebral disc degeneration to a certain extent. Additionally, the content of glycosaminoglycans and collagen in the nucleus pulposus of the intervertebral disc correlates with the FA and ADC values of the DTI parameters. Therefore, magnetic resonance DTI quantitative imaging provides a means to assess the biochemical composition changes within the intervertebral discs, offering valuable insights for the clinical diagnosis and evaluation of therapeutic efficacy in intervertebral disc degenerative diseases.
trial registrationChinese Clinical Trial Registry, ChiCTR2000041151, Registered on 19 December, 2020, https://www.chictr.org.cn/showproj.html?proj=206119 .
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.