Evidence map›Paper›PMID 39961826›Full record

ArticleAnalytical and bioanalytical chemistry2025

Self-protective DNAzyme-based dual-responsive three-way Y-probe for simultaneous determination of multiple pathogenic bacteria.

Bowen Li, Xinru Ren, Yijing Xiao, Weiqing Sun, Meili Yang, Tsing-Ao Pang, Rui Zhu, Zhiqiang Guo, Yu Wang, Su Liu and 1 more

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Article in Analytical and bioanalytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Bowen LiSchool of Biological Sciences and Technology, University of Jinan, Jinan, 250022, P. R. China.
Xinru RenKey Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, P. R. of China.
Yijing XiaoSchool of Water Conservancy and Environment, University of Jinan, Jinan, 250022, P. R. China.
Weiqing SunSchool of Water Conservancy and Environment, University of Jinan, Jinan, 250022, P. R. China.
Meili YangSchool of Biological Sciences and Technology, University of Jinan, Jinan, 250022, P. R. China.
Tsing-Ao PangSchool of Biological Sciences and Technology, University of Jinan, Jinan, 250022, P. R. China.
Rui ZhuSchool of Biological Sciences and Technology, University of Jinan, Jinan, 250022, P. R. China.
Zhiqiang GuoKey Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, P. R. of China.
Yu WangSchool of Biological Sciences and Technology, University of Jinan, Jinan, 250022, P. R. China.
Su LiuSchool of Water Conservancy and Environment, University of Jinan, Jinan, 250022, P. R. China.
Jiadong HuangSchool of Biological Sciences and Technology, University of Jinan, Jinan, 250022, P. R. China. chm_huangjd@ujn.edu.cn.

Funding

the National Natural Science Foundation of China 32072330
6 · The paper itself

Abstract

Foodborne pathogens, a major cause of foodborne illness due to their high virulence, pose a serious threat to public health. Consequently, identification of foodborne pathogens is essential for the prevention and treatment of foodborne infections. Consequently, there is an immediate need to establish a highly specific and precise approach for the concurrent detection of several foodborne pathogens. Herein, we developed a DNAzyme-based self-protecting dual-response nanoprobe for the simultaneous detection of two foodborne pathogens. The technique utilizes nanostructures to achieve logical signal input and output. In the presence of the target pathogen, the pathogen binds to the arch probe and releases the activation chain, which in turn activates a strand-displacement reaction and DNAzyme for signal amplification, producing different output signals to complete the simultaneous detection of multiple pathogens. The limits of detection for E. coli O157:H7 and S. typhimurium were determined to be 3.7 cfu/mL and 3.2 cfu/mL, with a measurement response time of 2 h. This approach enables ultrasensitive, specific, and simultaneous detection of two foodborne pathogens and is applicable for identifying foodborne pathogens in actual biological samples. The fluorescence detection of foodborne pathogens with a three-way Y-probe and DNAzyme coupling represents a novel approach for the concurrent identification of several foodborne diseases.

Indexed as

Biosensing TechniquesDNA, CatalyticEscherichia coli O157Food MicrobiologySalmonella typhimuriumFoodborne DiseasesLimit of DetectionDNA, CatalyticFoodborne pathogenLogic gateSimultaneous detectionUltrasensitive

Identifiers

PMID39961826

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.