ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Multidimensional Engineering of Escherichia coli for Efficient Adipic Acid Synthesis From Cyclohexane.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed.
- Reimagining Lignin Valorization: Synthetic Biology-Enabled Sustainable Aromatic Carbon Biomanufacturing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Engineering an Extremely Hybrid PKS for Adipic Acid Production.ACS synthetic biology · 2026Article
- Integrated biosynthesis of the lignan (-)-pluviatolide in resting and growingFrontiers in bioengineering and biotechnology · 2026Article
- Multidimensional Engineering of Escherichia coli for Efficient Adipic Acid Synthesis From Cyclohexane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Adipic acid (AA), a key aliphatic dicarboxylic acid, is conventionally manufactured through energy-intensive, multi-step chemical processes with significant environmental impacts. In contrast, biological production methods offer more sustainable alternatives but are often limited by low productivity. To overcome these challenges, this study reports the engineering of a single Escherichia coli for efficient biosynthesis of AA starting from cyclohexanol (CHOL), KA oil (mixture of CHOL and cyclohexanone (CHONE)), or cyclohexane (CH). To start with, a comprehensive screening of rate-limiting enzymes is conducted, particularly focusing on cytochrome P450 monooxygenase, followed by the optimization of protein expression using strategies such as protein fusion, promoter replacement, and genome editing. Consequently, an engineered E. coli capable of efficiently converting either KA oil or CH into AA is obtained, achieving remarkable product titers of 110 and 22.6 g L
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.