ArticlePeerJ2025
Activation of autophagy is required for clearance of mitochondrial ROS in patients with asthenozoospermia.
Article in PeerJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- The mitochondrial signature of sperm motility loss: a study of mitophagy and apoptosis in asthenozoospermia.Journal of assisted reproduction and genetics · 2026Article
- Bridging the Gap: How environmental exposures compromise male fertility and clinical Outlook.Current research in toxicology · 2026Review
- Colony-stimulating factor-1 as a potential therapeutic target in asthenozoospermia.Reproductive biology and endocrinology : RB&E · 2025Article
- Oxidative Damage Under Microgravity Conditions: Response Mechanisms, Monitoring Methods and Countermeasures on Somatic and Germ Cells.International journal of molecular sciences · 2025Review
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is regarded as an essential process for maintaining cell homeostasis. However, its role in the regulation of sperm motility is still less understood. In this study, we found that mitochondrial oxidative species (mROS) levels were elevated, but ATP levels and mitochondrial membrane potential (MMP) levels were reduced in human sperm with low motility (also called asthenozoospermia, AS) as compared to normal human sperm (normal sperm, NS). Immunocytochemistry staining showed that LC3 was mainly located in the neck of sperm. Western blot analysis showed that AS patients had elevated levels of the autophagy-related proteins LC3, Atg5, Atg7, and Beclin1. Flow cytometry showed that 3-MA treatment reduced sperm motility and MMP, but increased mROS. The results indicate that autophagy is essential for the clearance of mROS in sperm and the maintenance of mitochondrial function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.