Portable solutions for plant pathogen diagnostics: development, usage, and future potential.

The increasing prevalence of plant pathogens presents a critical challenge to global food security and agricultural sustainability. While accurate, traditional diagnostic methods are often time-consuming, resource-intensive, and unsuitable for real-time field applications. The emergence of portable diagnostic tools represents a paradigm shift in plant disease management, offering rapid, on-site detection of pathogens with high accuracy and minimal technical expertise. This review explores portable diagnostic technologies' development, deployment, and future potential, including handheld analyzers, smartphone-integrated systems, microfluidics, and lab-on-a-chip platforms. We examine the core technologies underlying these devices, such as biosensors, nucleic acid amplification techniques, and immunoassays, highlighting their applicability to detect bacterial, viral, and fungal pathogens in diverse agricultural settings. Furthermore, the integration of these devices with digital technologies, including the Internet of Things (IoT), artificial intelligence (AI), and machine learning (ML), is transforming disease surveillance and management. While portable diagnostics have clear advantages in speed, cost-effectiveness, and user accessibility, challenges related to sensitivity, durability, and regulatory standards remain. Innovations in nanotechnology, multiplex detection platforms, and personalized agriculture promise to further enhance the efficacy of portable diagnostics. By providing a comprehensive overview of current technologies and exploring future directions, this review underscores the critical role of portable diagnostics in advancing precision agriculture and mitigating the impact of plant pathogens on global food production.