ArticleLiver research (Beijing, China)2023
Optimization of high throughput spectral flow cytometry for immune cell profiling in mouse liver.
Article in Liver research (Beijing, China), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Maximizing Insights, Minimizing Animal Testing: A Framework for Validating Multiparametric Single-Cell Cytokine Analysis Panels.European journal of immunology · 2025Article
- Making deep immunophenotyping accessible: the successful application of a guided 23-parameter mouse immunophenotyping panel package provided through a shared resource.Frontiers in immunology · 2025Article
- Sphingosine kinase 2 (SphK2) depletion alters redox metabolism and enhances inflammation in a diet-induced MASH mouse model.Hepatology communications · 2024Article
- A GPCR-neuropeptide axis dampens hyperactive neutrophils by promoting an alternative-like polarization during bacterial infection.Immunity · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
The liver plays an important role in both metabolism and immunity. Disruption of the hepatic immune microenvironment is closely associated with various liver diseases. To gain a better understanding of how different types of immune cells contribute to the progression of liver diseases, it is crucial to thoroughly characterize hepatic immune cells. Although direct digestion of liver tissue is a relatively simple method for isolating immune cells, it often induces excessive hepatocyte death, which causes a release of intracellular components that leads to the activation of stress responses and injury in the surrounding cells. This injury can lead to excessive death in the hepatic immune cells, making isolation and accurate characterization of the immune profile challenging, especially in diseased livers. The method described here addresses these challenges by utilizing Phosphate buffered saline (PBS) and digestion buffer perfusions to eliminate contaminating blood cells, ensure a pure hepatic immune population, and minimize hepatic immune cell death. Further
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Registered trials
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