Evidence map›Paper›PMID 39956912›Full record

ArticleBMC infectious diseases2025

Parasitological and microbiological assessment of contact lens storage cases: a survey of asymptomatic lens student wearers from five medical specialties in Tunisia, North Africa.

Sameh Belgacem, Raja Chaâbane-Banaoues, Amira Mejri, Sawsen Ben Ifa, Maha Mastouri, Hamouda Babba

Abstract read
In one paragraph

Article in BMC infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Research on the effect ofFrontiers in microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sameh BelgacemLaboratory of Parasitology and Mycology (LP3M) LR12ES08, Faculty of Pharmacy of Monastir, Department of Clinical Biology B, Monastir, 5000, Tunisia.
Raja Chaâbane-BanaouesLaboratory of Parasitology and Mycology (LP3M) LR12ES08, Faculty of Pharmacy of Monastir, Department of Clinical Biology B, Monastir, 5000, Tunisia. rajachaabanebanaoues@gmail.com.ORCID https://orcid.org/0000-0002-3145-0165
Amira MejriLaboratory of Parasitology and Mycology (LP3M) LR12ES08, Faculty of Pharmacy of Monastir, Department of Clinical Biology B, Monastir, 5000, Tunisia.
Sawsen Ben IfaLaboratory of Microbiology, Fattouma Bourguiba University Hospital of Monastir, Monastir, 5000, Tunisia.
Maha MastouriLaboratory of Transmissible Diseases and Biologically Active Substances LR99ES27, Faculty of Pharmacy, Department of Clinical Biology B, University of Monastir, Monastir, 5000, Tunisia.
Hamouda BabbaLaboratory of Parasitology and Mycology (LP3M) LR12ES08, Faculty of Pharmacy of Monastir, Department of Clinical Biology B, Monastir, 5000, Tunisia.

Funding

Ministry of Higher Education and Scientific Research (Tunisia) (MESRS) LR12ES08 and LR99ES27 annual bugets
6 · The paper itself

Abstract

backgroundContamination of contact lenses has always been correlated with contamination of lenses and lens storage cases (LSCs), with higher loads of microorganisms in LSCs. The aim of the present study is to better understand non-compliance with strict hygiene rules in asymptomatic contact lens wearers, and to track circulating germs in LSCs that may affect the integrity of the eye.

methodsDemographic and behavioral data were collected from 111 asymptomatic lens wearers belonging to different medical faculties in Tunisia. Seventy LSCs were subjected to microbiological investigations, by direct examination and culture, in order to identify contaminating micro-organisms. The Richness and evenness of the species encountered were assessed to measure biodiversity on a local and international scale.

resultsThe study population was characterized by an average age of 22.8 ± 2.4 years and 95% female gender. Microbiological contamination accounted for 81.42% of LSCs, with only one case positive for Acanthamoeba spp. The Candida spp. fungal elements (20.0%) and Staphylococcus coagulase negative bacteria (60.5%) were the predominant microorganisms. Biodiversity markers namely: Simpson (0.802) and Shannon-Weiner (1.895) diversity indices were high in comparison to other studies. Monthly lens renewal (OR = 1.333, p = 0.040) and soft lens wear (OR = 4.66, p = 0.066) enhanced the installation of fungal elements.

conclusionsThe behaviors of contact lens wearers observed in this work corroborate those of all studies of contact lens wearers. The complexity of the recommended procedure and poor understanding of the instructions may explain any imperfections. This study highlights a high level of biodiversity in LSCs, and the strains in circulation are almost potentially pathogenic for humans.

Indexed as

Contact LensesEquipment ContaminationAdolescentAdultBacteriaBiodiversityFemaleFungiHumansMaleStudents, MedicalTunisiaYoung AdultBacteriaBiodiversity.\Contact lensFungiRisk factorsStorage cases

Identifiers

PMID39956912
PMCPMC11831826

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.