Evidence map›Paper›PMID 39953601›Full record

ArticlePorcine health management2025

Prevalence estimation of Pestivirus scrofae (atypical porcine pestivirus) among Hungarian pig herds and the effects of different sample types on detection rates.

Lilla Dénes, Mihály Albert, Barbara Igriczi, Gyula Balka

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Article in Porcine health management, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Lilla DénesDepartment of Pathology, University of Veterinary Medicine, István Str. 2., Budapest, 1078, Hungary. denes.lilla@univet.hu.
Mihály AlbertCEVA-Phylaxia (Ceva Sante Animale), Szállás Str. 5., Budapest, 1107, Hungary.
Barbara IgricziDepartment of Pathology, University of Veterinary Medicine, István Str. 2., Budapest, 1078, Hungary.
Gyula BalkaDepartment of Pathology, University of Veterinary Medicine, István Str. 2., Budapest, 1078, Hungary. balka.gyula@univet.hu.

Funding

Recovery and Resilience Facility RRF-2.3.1-21-2022-00001
6 · The paper itself

Abstract

backgroundAtypical porcine pestivirus (APPeV), also known as Pestivirus scrofae, is a member of the Pestivirus genus within the Flaviviridae family. Experimental infections have directly linked APPeV to congenital tremor (CT) type A-II in congenitally infected piglets born to challenged sows. Here, we report the assessment of the prevalence of APPeV in Hungarian pig herds and the influence of different sample types on detection rates.

resultsAltogether, 2650 blood serum, 198 oral fluid and 163 processing fluid samples were obtained via a systemic approach from 26 Hungarian farms and one Slovakian farm. The samples originated from different age groups and were analyzed via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The estimated prevalence of APPeV was determined to be 66.67% in the sampled farms, indicating the widespread distribution of the virus within Hungary. Within the positive farms, APPeV genetic material was detected in the serum (21%), processing fluid (57%), and oral fluid (72%) samples. Notably, in some farms, the presence of APPeV was confirmed in only specific sample types, and five farms had APPeV in all three sample types. Age group analysis revealed that 10-week-old animals had the highest positivity rate in their blood serum (27%), whereas 20-week-old animals presented the highest rate in their oral fluid samples (59%). Processing fluid and oral fluid samples proved to be valuable for noninvasive diagnostic matrices, allowing for efficient population-level virus detection. We determined the partial NS2-3 coding region of 15 Hungarian strains and a Slovakian strain, and our phylogenetic analysis revealed that very similar strains can be found on different farms.

conclusionIn conclusion, our study provides insights into APPeV prevalence in Hungarian pig herds, emphasizing the importance of different sample types for accurate diagnostics. These findings contribute to our understanding of the virus's distribution across different age groups.

Indexed as

Atypical porcine pestivirusGenetic variabilityNoninvasive sample typesViral prevalence

Identifiers

PMID39953601
PMCPMC11829520

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.