ArticleBiology direct2025
Functional improvement of natural Saccharomyces cerevisiae yeast strains by cell surface molecular engineering.
Article in Biology direct, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Development of a novel transformation system using zhongshengmycin inSynthetic and systems biotechnology · 2026Article
- Thiol release improvement in Saccharomyces cerevisiae oenological strains by CRISPR/Cas9-based IRC7 molecular cisgenesis.Journal of biological engineering · 2026Article
- A novel uranyl ion-binding peptide enhances U(VI) adsorption capacity and selectivity ofFrontiers in microbiology · 2026Article
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6 authors.
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Abstract
backgroundCellular boundaries of microorganisms can be modified by the expression in the cell wall of specific proteins endowed with relevant properties, improving their functional performance. So far, the surface display (SD) technique had been widely employed in the yeast Saccharomyces cerevisiae, but it was limited to few laboratory strains and never explored in sauvage strains, i.e., isolated from natural environment, which are featured by higher levels of genetic variability, leading to peculiar phenotypic traits of possible advantage in biotechnology.
resultsIn this work, a series of plasmids performing SD in natural yeast strains have been generated and further characterized by multiple functional and biochemical assays, providing the first experimental evidence that natural strains of S.cerevisiae can be genetically modified to express on their cell wall a protein-of-interest, which retains its biological competence. Interestingly, data further demonstrated that engineered strains expressing (transiently or stably) metal-binding proteins or peptides on cell surface exhibit significantly enhanced metal adsorption properties.
conclusionsThe molecular tools presented here can be very useful for yeast research community, as the plasmids efficiently support the surface engineering in virtually all S.cerevisiae strains, independently from either genetic background, source, or applications (wine, beer, bread). Overall, data strongly suggest that, upon genetic modification, S. cerevisiae strains isolated from natural environments could serve as promising platforms for biotechnological applications, as heavy metals removal or enzymes immobilization. Importantly, the strains investigated here represent only a small fraction of the multitude of S. cerevisiae strains present in nature yet to be isolated.
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