Evidence map›Paper›PMID 39953469›Full record

ArticleBMC pulmonary medicine2025

Development and validation of a Guinea pig model for concurrent allergic rhinitis and asthma using recombinant Der f 2.

Feng Xu, Li Hu, Jianzhong Wang, Lineng Zhang

Abstract readValidation Study
PubMed Full text at PMC Publisher
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Article in BMC pulmonary medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Feng XuDepartment of Otolaryngology-Head and Neck Surgery, Zhongshan Hospital of Fudan University, Shanghai, P. R. China.
Li HuDepartment of Otolaryngology-Head and Neck Surgery, Eye and ENT Hospital of Fudan University, Shanghai, P. R. China.
Jianzhong WangDepartment of Otolaryngology-Head and Neck Surgery, Zhongshan Hospital of Fudan University, Shanghai, P. R. China.
Lineng ZhangDepartment of Biotechnology and Biomedical Laboratory Sciences, Shanghai University of Medicine & Health Sciences Medical Technology College, Shanghai, P. R. China. 1482265969@qq.com.

Funding

Young Scholar Fund Project of Fudan University,China 2007
6 · The paper itself

Abstract

backgroundThis study aimed to develop a guinea pig model of concurrent allergic rhinitis and asthma.

methodsThirty-three guinea pigs were randomly divided into the control group and the experimental group. Guinea pigs in the experimental group were sensitized by intraperitoneal injection of recombinant wild-type Dermatophagoides farinae group 2 allergen (wt Der f 2) plus Al(OH)

resultsTwo guinea pigs with negative cutaneous reactions and three with less than 5 points of the nasal symptom assessment were excluded from the experimental group. The ACA test showed enhanced allergic reactions in the experimental group, and the quantification of extravasated Evans blue dye demonstrated significantly higher absorbance in the wt Der f 2 spots compared to mu Der f 2. Histologic analyses illustrated pathologic features typical of allergic rhinitis and asthma. MUC5AC levels in the nasal mucosa and lung samples were significantly higher in the experimental group than in the control group.

conclusionWe successfully established a guinea pig model of concurrent allergic rhinitis and asthma using a combination of sensitization, challenge, and validation methodologies with the allergen Der f 2, suitable for pathophysiological studies. CLINICAL TRIAL NUMBER: Not applicable.

Indexed as

Antigens, DermatophagoidesAsthmaDisease Models, AnimalRhinitis, AllergicAnimalsFemaleGuinea PigsLungMaleMucin 5ACNasal MucosaRecombinant ProteinsAntigens, DermatophagoidesMucin 5ACRecombinant ProteinsAllergic rhinitisAsthmaDer f 2Guinea pigInhalationIntraperitoneal administration

Identifiers

PMID39953469
PMCPMC11829409

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.