ArticleScientific reports2025
Application of graphene oxide in Agrobacterium-mediated genetic transformation and construction of a novel DNA delivery system for watermelon.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- An Efficient and Stable PEG-Mediated Transformation System for Medicinal FungusJournal of fungi (Basel, Switzerland) · 2026Article
- Waste Oyster Shell/Graphene Oxide Composite as a Dual-Functional Soil Conditioner and SRF: Impacts on Soil pH and Nutrient Availability.Nanomaterials (Basel, Switzerland) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Graphene oxide (GO) is widely used in biotechnology. The purpose of this study was to improve the efficiency of genetic transformation by constructing a delivery system based on GO. First, GO was applied in the traditional genetic transformation scheme for watermelons. We used hydroponics and tissue culture methods to determine the optimal concentration of GO for watermelon plant growth, we then used this concentration of GO for watermelon genetic transformation and found that GO can inhibit the growth of Agrobacterium tumefaciens and promote the growth of explants. This discovery can simplify the replacement of various culture media after explant infection, improve the regeneration rate of transgenic plants, and reduce experimental costs. To improve the efficiency of genetic transformation, a polymer-functionalized graphene oxide nanoparticle (GO-PEG-PEI) nanodelivery system was constructed, and the results showed that GO-PEG-PEI can transfer pCAMBIA1300-GFP plasmids into intact plant cells. We found that sheet-like GO-PEG-PEI can effectively load GFP and form small GO-PEG-PEI-GFP complexes, which can deliver pCAMBIA1300-GFP plasmids into plant cells. This research provides a new technique for molecular breeding.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.