Evidence map›Paper›PMID 39951437›Full record

ArticlePloS one2025

NOTCH1 promotes the elevation of GM-CSF and IL-6 through the EZH2/STAT3 pathway to facilitate the fibrotic state of the myocardium in DLBCL.

Huimin Zhang, Wen Dong, Lihong Zhang, Bing Ma, Jianying Wang, Yang Shen, Dan Zhao, Wanyi Yin, Yuexian Li, Qingchi Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Huimin ZhangHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.ORCID 0000-0001-7867-5997
Wen DongHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Lihong ZhangHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Bing MaHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Jianying WangHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Yang ShenHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Dan ZhaoHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Wanyi YinHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Yuexian LiHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Qingchi LiuHematology Department, The First Hospital of Hebei Medical University, Shijiazhuang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveInvestigate the role of the Neurogenic locus notch homolog protein 1 (NOTCH1) signaling pathway in Diffuse large B-cell lymphoma (DLBCL)-related heart pathogenesis.

methodsUtilize R (version 4.2.1) to retrieve DLBCL and myocardial infarction datasets from the GEO database, normalize data with limma, perform differential analysis and GO analysis with GOplot, and visualize findings with ggplot2. Various assays were conducted including stable cell line construction, myocardial infarction modeling, imaging, Western Blot, ELISA, staining, and functional assays.

resultsSignificant gene expression and pathway disparities were found between DLBCL and myocardial infarction samples. NOTCH1, The molecules named Recosomal-binding protein 70 (RBP-J), zeste 2 polycomb repressive complex 2 subunit (EZH2), trimethylated histone H3 at lysine 27 (H3K27me3), Signal Transducer And Activator Of Transcription 3 (STAT3) and Jumonji domain containing-3 (JMJD3) matters a lot in DLBCL. NOTCH1 inhibition decreased DLBCL cell proliferation and activity, reduced inflammatory factors, and improved myocardial fibrosis and infarction severity. NOTCH1 inhibits Granulocyte-macrophage colony-stimulating factor (GM-CSF) and Interleukin-6 (IL-6) expressions depending on STAT3 and EZH2. Co-culturing with DLBCL cells increased fibroblast proliferation, invasion, and fibrosis.

conclusionNOTCH1 signaling influences DLBCL development and myocardial infarction severity through the EZH2/STAT3 pathway, leading to increased heart fibrosis.

Indexed as

Enhancer of Zeste Homolog 2 ProteinGranulocyte-Macrophage Colony-Stimulating FactorInterleukin-6Lymphoma, Large B-Cell, DiffuseMyocardiumReceptor, Notch1STAT3 Transcription FactorAnimalsCell Line, TumorCell ProliferationFibrosisGene Expression Regulation, NeoplasticHumansMaleMiceMyocardial InfarctionEnhancer of Zeste Homolog 2 ProteinEZH2 protein, humanGranulocyte-Macrophage Colony-Stimulating FactorIL6 protein, humanInterleukin-6NOTCH1 protein, humanReceptor, Notch1STAT3 protein, humanSTAT3 Transcription Factor

Identifiers

PMID39951437
PMCPMC11828423

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.