ArticleArchives of microbiology2025
Standardizing biofilm quantification: harmonizing crystal violet absorbance measurements through extinction coefficient ratio adjustment.
Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- The phosphate specific transport system protein PhoU modulates persister cell formation of Klebsiella pneumoniae.Molecular genetics and genomics : MGG · 2026Article
- First Report and Integrated Characterization ofAnimals : an open access journal from MDPI · 2026Article
- 2'-Fucosyllactose AttenuatesMicroorganisms · 2026Article
- Investigating the Antimycobacterial, Antibiofilm, and Antioxidant Activities of Plant Extracts AgainstMicroorganisms · 2026Article
- Tolerance of outbreak-associated Candida parapsilosis isolates to antiseptics in a dry surface biofilm model.Scientific reports · 2026Article
- Dopamine Is a Key Regulatory Molecule forMicroorganisms · 2026Article
- Novel insights into the newly described Corynebacterium hesseae: first molecular and genomic characterization of a multidrug-resistant strain from blood culture.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Diversity and Functional Potential of Yeasts Inhabiting Honey Bee Drones.Microorganisms · 2025Article
- Biofilm-mediated resistance to berberine inFrontiers in cellular and infection microbiology · 2025Article
- Monitoring of Staphylococcus epidermidis biofilm formation on platelet storage bag surfaces.PloS one · 2025Article
- Study on resistance mechanisms and molecular epidemiology of carbapenem-resistantFrontiers in cellular and infection microbiology · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precise quantification of biofilm is critical as the formation and persistence of biofilm have significant implications in the environmental, therapeutic and industrial contexts. The microtiter plate assay using crystal violet with 33% glacial acetic acid or 94-100% ethanol as the resolubilising agent is widely used for the categorisation of biofilms into weak, moderate and strong categories. But, the use of varying wavelengths for the measurement of biofilm resulted in discrepancies in categorisation across the studies due to the difference in the extinction coefficient of CV. This study emphasises the importance of measuring the biofilm at the absorbance maximum (λ
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.