Evidence map›Paper›PMID 39949397›Full record

ArticleFrontiers in pharmacology2024

FAM134B-mediated endoplasmic reticulum autophagy protects against cisplatin-induced spiral ganglion neuron damage.

Fan Wang, Yue Xu, Yajie Wang, Qian Liu, Yanan Li, Weiwei Zhang, Huiming Nong, Junhong Zhang, Hao Zhao, Huaqian Yang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in pharmacology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Fan WangDepartment of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, China.
Yue XuDepartment of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, China.
Yajie WangDepartment of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Qian LiuDepartment of Otolaryngology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Yanan LiDepartment of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Weiwei ZhangDepartment of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, China.
Huiming NongDepartment of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Junhong ZhangDepartment of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Hao ZhaoDepartment of Otolaryngology, Head and Neck Surgery, People's Hospital, Peking University, Beijing, China.
Huaqian YangCyrus Tang Medical Institute, Soochow University, Suzhou, China.
Lingchuan GuoDepartment of Pathology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Jianfeng LiDepartment of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, China.
Hong LiDepartment of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital, Shandong University, Jinan, China.
Qianqian YangDepartment of Pathology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Cochlear spiral ganglion neurons (SGNs) could be damaged by ototoxic drug, cisplatin (Cis), during which process autophagy was involved. FAM134B, the first detected endoplasmic reticulum autophagy (ER-phagy) receptor, plays an important part in the dynamic remodelling of the ER, the mutation of which affects sensory and autonomic neurons. However whether FAM134B-mediated ER-phagy involved in Cis-induced SGN damage or not was unknown. The present study was designed to determine whether FAM134B is expressed in SGNs of C57BL/6 mice and, if so, to explore the potential function of FAM134B in Cis-induced SGN damage Methods: Middle turns of neonatal murine cochleae were cultured and treated with 30 μM Cis Results: We first found the expression of FAM134B in the cytoplasm of SGNs, especially in the fourth postnatal day mice. Results showed decreases in the number of SGNs and FAM134B expression, as well as increases of ROS level, ER stress, ER-phagy, and apoptosis after Cis stimulus. Inhibiting autophagy increased the expression of FAM134B, and aggravated Cis-induced SGN damage, while the opposite changes were observed when autophagy was activated. Additionally, co-treatment with the N-Acetyl-L-Cysteine (NAC), ROS scavenger, alleviated Cis-induced ER stress, ER-phagy, and apoptosis. What's more, knockdown the expression of FAM134B in SGNs made SGNs more vulnerable to cisplatin-induced injury. Discussion: The present study revealed the expression pattern of FAM134B in C57BL/6 murine SGNs for the first time. Moreover, our work further verified the protective function of FAM134B mediated by ER-phagy in Cis-induced SGN apoptosis, at least partially, correlated with the accumulation of ROS and induction of ER stress, though the detailed regulatory mechanism through which needs much more work to reveal.

Indexed as

cisplatinER-phagyER stressFAM134BSGNS

Identifiers

PMID39949397
PMCPMC11821923

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.