Evidence map›Paper›PMID 39948619›Full record

ArticleCell communication and signaling : CCS2025

HIF1α controls steroidogenesis under acute hypoxic stress.

Stephen Ariyeloye, Deepika Watts, Mangesh T Jaykar, Cagdas Ermis, Anja Krüger, Denise Kaden, Barbara K Stepien, Vasileia Ismini Alexaki, Mirko Peitzsch, Nicole Bechmann and 3 more

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Article in Cell communication and signaling : CCS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Stephen Ariyeloye *Institute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Deepika Watts *Institute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Mangesh T JaykarInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Cagdas ErmisInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Anja KrügerInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Denise KadenInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Barbara K StepienInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Vasileia Ismini AlexakiInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Mirko PeitzschInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Nicole BechmannInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Peter MirtschinkInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany.
Ali El-ArmoucheDepartment of Pharmacology and Toxicology, Faculty of Medicine, Technische Universität Dresden, Dresden, 01307, Germany.
Ben WielockxInstitute of Clinical Chemistry and Laboratory Medicine, University Carl Gustav Carus and Medical Faculty, Technische Universität Dresden, Fetscherstrasse 74, Dresden, 01307, Germany. Ben.Wielockx@tu-dresden.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHypoxia is a critical physiological and pathological condition known to influence various cellular processes, including steroidogenesis. While previous studies, including our own, have highlighted the regulatory effects of Hypoxia-Inducible Factor 1α (HIF1α) on steroid production, the specific molecular mechanisms remain poorly understood. This study investigates the role of hypoxia and HIF1α in steroid biosynthesis across multiple experimental models during acute exposure to low oxygen levels.

methodsTo assess the extent to which acute hypoxia modulates steroidogenesis, we employed several approaches, including the Y1 adrenocortical cell line, and a conditional HIF1α-deficient mouse line in the adrenal cortex. We focused on various regulatory patterns that may critically suppress steroidogenesis.

resultsIn Y1 cells, hypoxia upregulated specific microRNAs in a HIF1α-dependent manner, resulting in the suppression of mRNA levels of critical steroidogenic enzymes and a subsequent reduction in steroid hormone production. The hypoxia/HIF1α-dependent induction of these microRNAs and the consequent modulation of steroid production were confirmed in vivo. Notably, using our adrenocortical-specific HIF1α-deficient mouse line, we demonstrated that the increase in miRNA expression in vivo is also directly HIF1α-dependent, while the regulation of steroidogenic enzymes (e.g., StAR and Cyp11a1) and steroid production occurs at the level of protein translation, revealing an unexpected layer of control under hypoxic/HIF1 α conditions in vivo.

conclusionsThese findings elucidate the molecular mechanisms underlying acute hypoxia/HIF1α-induced changes in steroid biosynthesis and may also be useful in developing new strategies for various steroid hormone pathologies.

Indexed as

Hypoxia-Inducible Factor 1, alpha SubunitSteroidsAdrenal CortexAnimalsCell HypoxiaCell LineMiceMicroRNAsHif1a protein, mouseHypoxia-Inducible Factor 1, alpha SubunitMicroRNAsSteroidsAdrenal glandHypoxia-inducible factorsMicroRNAOxygen sensorsSteroidogenesis

Identifiers

PMID39948619
PMCPMC11827267

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.