Evidence map›Paper›PMID 39948287›Full record

ArticleMikrochimica acta2025

Terminal deoxynucleotidyl transferase combined dual-toehold catalytic hairpin assembly for sensitive sperm DNA integrity evaluation.

Jiezhen Pan, Lifeng Zhang, Yuxin Liang, Qingcong Luo, Xiaohe Zhang, Ye Zhang, Shihua Luo

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Jiezhen Pan *Center for Clinical Laboratory Diagnosis and Research, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise , Guangxi, 533000, China.
Lifeng Zhang *Department of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, P. R. China.
Yuxin LiangDepartment of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, P. R. China.
Qingcong LuoDepartment of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, P. R. China.
Xiaohe ZhangDepartment of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, P. R. China.
Ye ZhangDepartment of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, P. R. China. zhangye232@i.smu.edu.cn.
Shihua LuoCenter for Clinical Laboratory Diagnosis and Research, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise , Guangxi, 533000, China. luosh@ymun.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A simple and sensitive electrochemical biosensor was proposed using terminal deoxynucleotidyl transferase (TdT) and dual-toehold catalytic hairpin assembly (dtCHA) for sperm DNA fragmentation analysis. The TdT extends the polyA sequences at the 3'-OH end, which triggers the dtCHA reaction and amplifies the signal, allowing for efficient measurement of the number of 3'-OH groups (equal to the quantity of breakpoints). The proposed system showed a broad linear range from 10 fM to 10 nM, with a detection limit of 7.7 fM. Furthermore, the results from clinical samples indicated that the mean quantity of DNA breakpoints (MDB) correlated more strongly with sperm motility than the commonly used DNA fragmentation index (DFI). Therefore, the platform can provide an alternative tool for sperm DNA fragmentation analysis, offering significant potential for clinical diagnosis.

Indexed as

Biosensing TechniquesDNADNA FragmentationDNA NucleotidylexotransferaseSpermatozoaElectrochemical TechniquesHumansInverted Repeat SequencesLimit of DetectionMaleDNADNA NucleotidylexotransferaseDifferential pulse voltammetryDual-toehold catalytic hairpin assemblyElectrochemical biosensorMean quantity of DNA breakpointsSperm DNA fragmentation analysisTerminal deoxynucleotidyl transferase

Identifiers

PMID39948287

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.