Evidence map›Paper›PMID 39948206›Full record

ArticleVirus genes2025

Study of Hsp90α and Hsp90β role in virus replication using cell lines with Hsp90 gene knockouts.

Pawel Bieganowski, Iga Dalidowska, Olga Gazi, Magdalena Guzowska, Maciej Przybylski

Abstract read
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In one paragraph

Article in Virus genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Pawel BieganowskiMossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland.
Iga DalidowskaMossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland.
Olga GaziChair and Depatment of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland.
Magdalena GuzowskaDivision of Biochemistry and Dietetics, Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
Maciej PrzybylskiChair and Depatment of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland. maciej.przybylski@wum.edu.pl.

Funding

European Science Foundation POWR.03.02.00-00-I028/17-00Narodowe Centrum Nauki 2016/23/B/NZ6/02536
6 · The paper itself

Abstract

Replication of the human Enterovirus 71 (EV71) and herpes simplex virus 1 (HSV-1) requires Hsp90 chaperone activity. Vertebrate cells express two cytosolic Hsp90 proteins, Hsp90α and Hsp90β. Earlier reports suggested that EV71 replication might depend solely on the Hsp90β, whereas HSV-1 replication depended on Hsp90α. Here, we describe construction of the cell line knockouts missing Hsp90α or Hsp90β protein. Using these cells, we found that HSV-1 and, another enterovirus, Coxsackievirus B5 (CVB5) replicate in both Hsp90α and Hsp90β knockout cells with equal efficiency. The presented results demonstrate that cell lines with a mutation inactivating the specific HSP90 gene might be an easy-to-use and robust system to study specific cellular functions of Hsp90α and Hsp90β.

Indexed as

Enterovirus A, HumanHerpesvirus 1, HumanHSP90 Heat-Shock ProteinsVirus ReplicationAnimalsCell LineChlorocebus aethiopsGene Knockout TechniquesHumansHSP90AA1 protein, humanHSP90AA2P protein, humanHSP90AB1 protein, humanHSP90 Heat-Shock ProteinsCoxsackievirusCRISPR/Cas9Herpes simplex virus 1Hsp90 alphaHsp90 betaKnockout

Identifiers

PMID39948206

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.