Evidence map›Paper›PMID 39947999›Full record

ArticleThe journal of prevention of Alzheimer's disease2025

The impact of pre-analytical factors on plasma biomarkers for Alzheimer's disease: The ASPREE Healthy Ageing Biobank.

Zimu Wu, Michelle M Mielke, Anne M Murray, James Phung, Alice Owen, Robyn L Woods, Danni Li, Jo Wrigglesworth, Joanne Ryan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of prevention of Alzheimer's disease, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zimu WuSchool of Public Health and Preventive Medicine, Monash University, Melbourne, VIC 3004, Australia. Electronic address: zimu.wu1@monash.edu.
Michelle M MielkeDepartment of Epidemiology and Prevention, School of Medicine, Wake Forest University, Winston-Salem, NC 27101, USA.
Anne M MurrayDivision of Geriatric and Palliative Medicine, Department of Medicine, Hennepin Healthcare, Minneapolis, Minnesota 55404, USA; Berman Center for Outcomes and Clinical Research, Hennepin Healthcare Research Institute, Minneapolis, Minnesota 55404, USA.
James PhungSchool of Public Health and Preventive Medicine, Monash University, Melbourne, VIC 3004, Australia.
Alice OwenSchool of Public Health and Preventive Medicine, Monash University, Melbourne, VIC 3004, Australia.
Robyn L WoodsSchool of Public Health and Preventive Medicine, Monash University, Melbourne, VIC 3004, Australia.
Danni LiDepartment of Lab Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota 55404, USA.
Jo WrigglesworthSchool of Public Health and Preventive Medicine, Monash University, Melbourne, VIC 3004, Australia.
Joanne RyanSchool of Public Health and Preventive Medicine, Monash University, Melbourne, VIC 3004, Australia.

Funding

ASPirin in Reducing Events in the ElderlyU01AG029824 · NIA · HENNEPIN HEALTHCARE RESEARCH INSTITUTE · PI MCNEIL, JOHN JAMES, MURRAY, ANNE M · 2009 to 2018
$64.6M
Main Administrative CoreU19AG062682 · NIA · HENNEPIN HEALTHCARE RESEARCH INSTITUTE · PI CHAN, ANDREW T, MURRAY, ANNE M · 2019 to 2023
$42.9M
Wake Forest University School of Medicine Alzheimer's Disease Research CenterP30AG072947 · NIA · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI SUZANNE CRAFT · 2021 to 2026
$24.3M
Sex-specific risk factors and trajectories of blood biomarkers for Alzheimer's disease and related dementiasRF1AG079397 · NIA · HENNEPIN HEALTHCARE RESEARCH INSTITUTE · PI MIELKE, MICHELLE M, MURRAY, ANNE M · 2022 to 2024
$4.5M
NIA NIH HHS P30 AG072947NIA NIH HHS RF1 AG079397NIA NIH HHS U01 AG029824NIA NIH HHS U19 AG062682
6 · The paper itself

Abstract

backgroundThe conditions under which samples were collected, processed, and stored in biobanks may influence Alzheimer's disease (AD) biomarker levels.

objectivesThis study aims to investigate whether a range of pre-analytical factors influence plasma levels of AD biomarkers.

methodsData were obtained from the ASPREE Healthy Ageing Biobank, a cohort of healthy community-dwelling older individuals aged 70+ years in Australia. Five biomarkers were measured using plasma from 11,868 individuals: phosphorylated-tau181 (p-tau181), neurofilament light chain (NfL), glial fibrillary acidic protein (GFAP), and amyloid-beta 42 and 40 (Aβ42/Aβ40). Linear regression examined the association between pre-analytical factors and biomarker levels.

resultsParticipants were aged 70-96 years, and 54 % were female. The mean storage time for samples was 10.6 years (range: 7.7-13.5). Some significant associations were identified between pre-analytical factors and biomarkers, in particular for p-tau181, but the effect sizes were small. Weak negative associations were found between p-tau181 and the time from venepuncture to laboratory (transport) (β: -0.82, p = 0.03), laboratory processing to frozen storage (β:-1.56, p < 0.001), and total years of storage (β: -0.45, p = 0.007), while a positive association was found with intermediate storage at -20 °C/-30 °C compared to -80 °C (β: 2.24, p = 0.004). Longer fasting time was associated with higher levels of both NfL (β: 0.15, p < 0.001) and GFAP (β: 1.75, p < 0.001).

conclusionFollowing standard operating procedures, AD biomarkers can be measured in plasma from biobanks stored for up to 13 years, with minimal impact from long-term storage or other pre-analytical factors.

Indexed as

Alzheimer DiseaseBiological Specimen BanksBiomarkersSpecimen HandlingAgedAged, 80 and overAmyloid beta-PeptidesAustraliaFemaleGlial Fibrillary Acidic ProteinHumansMaleNeurofilament ProteinsPeptide Fragmentstau ProteinsAmyloid beta-PeptidesBiomarkersGFAP protein, humanGlial Fibrillary Acidic Proteinneurofilament protein LNeurofilament ProteinsPeptide Fragmentstau ProteinsAlzheimer's diseaseAmyloidDementiaPlasma biomarkersPre-analytical factorsTau

Identifiers

PMID39947999
PMCPMC12183983

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.