Evidence map›Paper›PMID 39946333›Full record

ArticlePloS one2025

Relationship between chromatin configuration and maturation ability of rat oocytes in vitro and in vivo.

Zhaoqing Gong, Yujie Wang, Jiayi Tang, Yang Xu, Hongkai Wang, Yimiao Zhang, Lixin Xiong, Changzheng Sun, Yiyang Li, Yan Yang and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Zhaoqing GongCollege of Basic Medical Science, Hebei University, Baoding, Heibei Province, P. R China.
Yujie WangDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Jiayi TangDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Yang XuDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Hongkai WangDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Yimiao ZhangDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Lixin XiongDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Changzheng SunDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Yiyang LiDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Yan YangMorphological Laboratory, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Minhua YaoDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Heng CaiDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Zengshuo ManDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Siyu XuanDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Yangyang TangDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Ziao ZhaoDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Jiaxin SunDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Dongwei LiuDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Yanping SuDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Xinghua XuDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.
Mingjiu LuoShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, Shandong Province, P. R China.
Hongshu SuiDepartment of Histology and Embryology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, Shandong Province, P. R China.ORCID 0000-0002-1671-277X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeEmbryo engineering requires a large number of oocytes, which undergo in vitro maturation (IVM). Understanding how to select the best quality oocytes is key to improving IVM efficiency. Oocytes have different germinal vesicle (GV) chromatin configurations, which may explain the heterogeneity in oocyte quality during IVM. However, no reports have categorized, the chromatin configuration of rat GVs or evaluated, the association between the chromatin configuration and oocytes development.

methodsThe GV chromatin configuration of rat oocytes was divided into seven types according to the degree of chromatin compaction: non-surrounded nucleolus (NSN), prematurely condensed NSN, partly NSN, partly surrounded nucleolus (SN-1), SN-1, condensed SN-1, and aggregated (SN-2). The chromatin configuration distribution was compared during the different stages of oocyte growth and maturation. We also analyzed the changes in the chromatin configuration at different GV stages during IVM. Moreover, the factors affecting the chromatin configuration were analyzed.

resultsThe SN-2 configuration increased with rat oocyte growth and maturation, suggesting that SN-2 facilitates oocyte development. RNA transcription activity in rat oocyte GVs was inversely correlated with oocyte IVM.

conclusionsThe SN-2 chromatin configuration was related to rat oocyte growth and maturation. RNA transcription activity in rat oocytes in the GV stage was inversely correlated with oocyte maturation.

Indexed as

ChromatinIn Vitro Oocyte Maturation TechniquesOocytesAnimalsCell NucleolusFemaleRatsChromatin

Identifiers

PMID39946333
PMCPMC11825056

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