Evaluation of the Effect of Recombinant Human Angiogenin on the Functional Properties of EA.Hy926 Endothelial Cells and Human Skin Fibroblasts In Vitro.

A P Lykov, A M Goryachkin, M A Surovtseva, M N Drovosekov, O V Poveshchenko

Abstract read

In one paragraph

Article in Bulletin of experimental biology and medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

A P LykovResearch Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia. aplykov2@mail.ru.

A M GoryachkinNovosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia.

M A SurovtsevaResearch Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

M N DrovosekovNovosibirsk State Medical University, Ministry of Health of the Russian Federation, Novosibirsk, Russia.

O V PoveshchenkoResearch Institute of Clinical and Experimental Lymphology - Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We studied the effects of recombinant human angiogenin on in vitro proliferation, migration, apoptosis, and secretion of human skin fibroblasts (FB) and endothelial cells (EC; EA.Hy926). The most potent stimulation of migration and proliferation of EC and FB was observed at angiogenin concentration of 125 ng/ml in a serum-free medium. Angiogenin had little effect on apoptosis/necrosis of EC and FB. It promoted IL-1β production by EC in a serum-free medium and did not affect FB. Angiogenin had the opposite effects on the production of TNFα and IL-10 by cells: it suppressed the production of these factors by EC in a serum-free medium and enhanced their production by FB in media with and without serum. In a serum-free medium, VEGF production by EC increased and by FB drops. NO production by EC in response to angiogenin stimulation did not depend on the culturing conditions, while NO production by FB increased in complete culture media. Thus, the presence of FCS in the culture medium considerably modulated the effect of angiogenin.

Indexed as

Endothelial CellsFibroblastsRibonuclease, PancreaticApoptosisCell LineCell MovementCell ProliferationCulture Media, Serum-FreeHumansInterleukin-10Interleukin-1betaNitric OxideRecombinant ProteinsSkinTumor Necrosis Factor-alphaVascular Endothelial Growth Factor AangiogeninCulture Media, Serum-FreeInterleukin-10Interleukin-1betaNitric OxideRecombinant ProteinsRibonuclease, PancreaticTumor Necrosis Factor-alphaVascular Endothelial Growth Factor Aangiogenincytokinesendothelial cellsfibroblasts

Identifiers

PMID39945952

DOI10.1038/sj.onc.1208223

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.