Evidence map›Paper›PMID 39945949›Full record

ArticleBulletin of experimental biology and medicine2025

Immunochemical Characteristics and Functional Activity of Monoclonal Antibodies Obtained to the Recombinant Form of Pneumolysin.

I V Yakovleva, N F Gavrilova, E A Kurbatova, D S Vorobyev, E S Petukhova, I B Semenova, A E Zaitsev, Yu V Volokh, A Yu Leonova, A V Poddubikov

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Article in Bulletin of experimental biology and medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

I V YakovlevaI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
N F GavrilovaI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
E A KurbatovaI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia. kurbatova6162@yandex.ru.
D S VorobyevI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
E S PetukhovaI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
I B SemenovaI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
A E ZaitsevI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Yu V VolokhI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
A Yu LeonovaI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
A V PoddubikovI. Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monoclonal antibodies (mAbs) of the IgG1 isotype obtained to the full-length recombinant pneumolysin (rPly) did not recognize or poorly recognized conformational epitopes of native pneumolysin in ELISA. At the same time, polyclonal antibodies (pAbs(rPly)) detected native pneumolysin in sandwich ELISA when they were used as capture and detecting antibodies simultaneously. All mAbs(rPly) inhibited erythrocyte hemolysis induced by native pneumolysin. The combined use of mAbs(rPly) did not reveal an increase in optical density in ELISA and an increase in inhibition hemolytic activity, which suggests that mAbs(rPly) are directed to the same or spatially closely located epitopes. pAbs(rPly) more effectively inhibited erythrocyte hemolysis than mAbs(rPly).

Indexed as

Antibodies, MonoclonalBacterial ProteinsStreptolysinsAnimalsEnzyme-Linked Immunosorbent AssayEpitopesErythrocytesHemolysisHumansImmunoglobulin GMiceRecombinant ProteinsStreptococcus pneumoniaeAntibodies, MonoclonalBacterial ProteinsEpitopesImmunoglobulin GplY protein, Streptococcus pneumoniaeRecombinant ProteinsStreptolysinsinhibition of hemolytic activitymonoclonal antibodiesnative pneumolysinrecombinant pneumolysinsandwich ELISA

Identifiers

PMID39945949

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.