Analysis of the Alkaline Resistance Mechanism of Halomonas alkalicola CICC 11012 s by Proteomics and Metabolomics.

In recent years, as excellent industrial microorganisms, Halomonas has become a potential chassis cell of the next generation of industrial biotechnology because of its advantages of low complexity, antipollution ability, and rapid fermentation. Therefore, there is an urgent need to study the genome information, synthetic biology, multiomics, and other technologies of Halomonas, and it is also highly important to study its tolerance to extreme environments. Halomonas alkalicola CICC 11012 s is the most alkaliphilic bacterium in the genus Halomonas and is an excellent alkali-resistant bacterium that was independently isolated in our laboratory; this bacterium plays a certain role in industrial pollution control and the application of synthetic biology chassis cells. The H. alkalicola mutant was designed and constructed via CRISPR technology in the early stage of this experiment, which verified that the tonb gene plays an important role in the alkali resistance mechanism of this strain. Therefore, the molecular mechanism of the response of H. alkalicola CICC 11012 s to alkaline stress was explored through combined proteomic and metabolomic analysis. The experimental results revealed that the wild-type and mutant strains evolved multilevel adaptive strategies to regulate pH homeostasis in response to alkaline stress, including increasing their membrane transport activities and synthesizing carbohydrates and amino acids. In summary, the experimental results provide a deep understanding of the alkaline response mechanism of alkalophilic bacteria, thereby further promoting their application in different environments.