Evidence map›Paper›PMID 39945694›Full record

ArticleCytotherapy2025

Acute respiratory distress vs healthy lung environments differently affect mesenchymal stromal cell extracellular vesicle miRNAs.

Sara Rolandsson Enes, Irakli Dzneladze, Thomas H Hampton, Samuel L Neff, Lori Asarian, Jayita Barua, Tobias Tertel, Bernd Giebel, Nicolas Pereyra, David H McKenna and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytotherapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Sara Rolandsson EnesDepartment of Medicine, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA; Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden. Electronic address: sara.rolandsson_enes@med.lu.se.
Irakli DzneladzeInterdepartmental Division of Critical Care, Department of Medicine and the Keenan Center for Biomedical Research, St. Michael's Hospital, University of Toronto, Toronto, Canada.
Thomas H HamptonDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.
Samuel L NeffDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.
Lori AsarianDepartment of Medicine, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
Jayita BaruaDepartment of Medicine, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
Tobias TertelInstitute of Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen Germany.
Bernd GiebelInstitute of Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen Germany.
Nicolas PereyraDepartment of Biochemistry and Molecular Biology, The University of British Columbia, Canada, The University of British Columbia Centre for Blood Research, Vancouver, Canada.
David H McKennaDepartment of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, USA.
Pingzhao HuDepartment of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada.
Erica ActonInterdepartmental Division of Critical Care, Department of Medicine and the Keenan Center for Biomedical Research, St. Michael's Hospital, University of Toronto, Toronto, Canada.
Alix AshareDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA; Section of Pulmonary and Critical Care Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA.
Kathleen D LiuDepartments of Medicine and Anesthesiology and the Cardiovascular Research Institute, University of California San Francisco.
Anna D KrasnodembskayaWellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry, and Biomedical Sciences, Queens University, Belfast, UK.
Karen EnglishCellular Immunology Laboratory, Biology Department, Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. Kildare, Ireland.
Bruce A StantonDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA.
Patricia R M RoccoLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro; National Institute of Science and Technology for Regenerative Medicine, Rio de Janeiro, Brazil.
Michael A MatthayDepartments of Medicine and Anesthesiology and the Cardiovascular Research Institute, University of California San Francisco.
Claudia C Dos SantosInterdepartmental Division of Critical Care, Department of Medicine and the Keenan Center for Biomedical Research, St. Michael's Hospital, University of Toronto, Toronto, Canada.
Daniel J WeissDepartment of Medicine, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.

Funding

Translational Research CoreP30DK117469 · NIDDK · DARTMOUTH COLLEGE · PI DEBORAH A HOGAN · 2018 to 2026
$13.9M
Retrieval, Reprocessing, Normalization and Sharing of Gene Expression and Lung Microbiome Data Sets to Facilitate AI/ML Analysis Studies of Bacterial Lung InfectionsR01HL151385 · NHLBI · DARTMOUTH COLLEGE · PI STANTON, BRUCE A. · 2021 to 2024
$2.4M
Optimizing Functional Recellularization of Acellular Human Lung ScaffoldsR01HL127144 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI WEISS, DANIEL J · 2015 to 2018
$1.5M
The Role of Macrophage Metabolic Crosstalk in CF Chronic Lung InflammationR56HL158923 · NHLBI · DARTMOUTH-HITCHCOCK CLINIC · PI ASHARE, ALIX · 2023 to 2023
$637k
Decellularized Avian Lungs for Use in Pulmonary TherapeuticsR21EB024329 · NIBIB · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI WEISS, DANIEL J · 2017 to 2018
$428k
NHLBI NIH HHS R01 HL127144NHLBI NIH HHS R01 HL151385NHLBI NIH HHS R56 HL158923NIBIB NIH HHS R21 EB024329NIDDK NIH HHS P30 DK117469
6 · The paper itself

Abstract

The acute respiratory distress syndrome (ARDS) inflammatory environment alters mesenchymal stromal cell (MSC) gene and protein expression but effects on microRNA (miRNA) content of MSC-extracellular vesicle (EVs) remain unknown. To assess this, sequencing analysis of EV-miRNAs prepared from human bone marrow-derived MSCs (hMSCs) exposed ex vivo to bronchoalveolar lavage fluid (BALF) from ARDS patients or healthy volunteers (HV) identified a number of differentially expressed miRNAs. Discriminant, differential expression, and functional enrichment analyses identified 14 miRNAs significantly changed following ARDS versus HV BALF exposure. Network analysis showed 4 (miR-760, miR-3175, miR-885-3p, and miR-766-3p) of the 14 EV-miRNAs formed a regulatory "hub", suggesting co-targeting of specific gene pathways. In silico prediction identified a number of pathways important in lung injury. Two miRNAs involved in regulation of the cystic fibrosis transmembrane conductance regulator (CFTR), miRNA-145-5p and miRNA-138-5p, were also significantly increased in ARDS BALF-exposed hMSCs EVs. Functionally, EVs from hMSCs exposed to either ARDS or HV BALF had differential effects on CFTR Cl

Indexed as

Extracellular VesiclesLungMesenchymal Stem CellsMicroRNAsRespiratory Distress SyndromeBronchoalveolar Lavage FluidCystic Fibrosis Transmembrane Conductance RegulatorFemaleHumansMaleMiddle AgedCystic Fibrosis Transmembrane Conductance RegulatorMicroRNAsacute respiratory distress syndromebronchoalveolar lavage fluidCFTRexosomesextracellular vesiclesmesenchymal stromal cellsmiRNA

Identifiers

PMID39945694
PMCPMC13181138

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.