ArticlePest management science2025
Comparative transcriptomics reveals different profiles between diflubenzuron-resistant and -susceptible phenotypes of the mosquito Culex pipiens.
Article in Pest management science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Allelic Survey and Population Genomics in a Hotspot of Diflubenzuron Resistance in the MosquitoInsects · 2026Article
- Assessment of the Impact of Diflubenzuron on the Inhibition of Adult Emergence inJournal of arthropod-borne diseases · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
Abstract
backgroundChitin-synthesis inhibitors (CSIs) represent a major tool in vector control. The intensive use of these compounds has led to the evolution of resistance against several CSIs, including diflubenzuron (DFB). DFB resistance has been associated to a target-site mechanism; however, studies investigating the gene expression profile of resistant phenotypes are limited, preventing a full understanding of DFB resistance. Here, we analyzed the constitutive gene expression of susceptible and DFB-resistant individuals of the mosquito Culex pipiens, a major disease vector in temperate areas.
resultsComparative gene expression analysis between susceptible and DFB-resistant individuals identified 527 differentially expressed genes (i.e., 432 up-regulated and 95 down-regulated genes). Among the up-regulated genes, 87 genes belong to gene families associated with insecticide resistance in arthropods, such as cytochrome P450s, glutathione-S-transferases, UDP-glucuronosyltransferases, heat shock proteins and cuticular proteins (CPs). Interestingly, the CP transcripts were the most abundant among up-regulated genes (73 of 87), and furthermore they constitute 11 of the 20 most over expressed genes. The enrichment of transcripts associated with cuticle synthesis was also identified by the Gene Ontology (GO) enrichment analysis.
conclusionsAdaptation to insecticides can involve transcriptional changes in genes encoding for multiple defense mechanisms. Our results identified the over-expression of transcripts associated with detoxification and cuticle synthesis in DFB-resistant individuals of Cx. pipiens. Multiple mechanisms, beyond the known target-site mechanism, may therefore contribute to the DFB-resistant phenotype. Together these findings corroborate the complexity underpinning the resistant phenotypes and provide important information for the implementation of effective control strategies against mosquito vectors. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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