ArticleAnimals : an open access journal from MDPI2025
Antioxidant Synergy in a Mixture of Powder Plant Leaves and Effects on Metabolic Profile, Oxidative Status and Intestinal Morpho-Histochemical Features of Laying Hens.
Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- An Integrated Approach to Evaluate the Influence of DietaryAnimals : an open access journal from MDPI · 2026Article
- Milk Yield and Quality, Metabolic Profile and Oxidative Status in Lactating Goats, as Affected by Silage Based on Agro-Industrial By-Products.Animals : an open access journal from MDPI · 2026Article
- Effects of Dietary Ginger (Animals : an open access journal from MDPI · 2025Article
- Morphology and Glycan Composition of the Mandibular Glands in the White-Eared Opossum (Didelphis albiventris).Journal of morphology · 2025Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Phenolic antioxidant intake is encouraged to prevent oxidative damage, and antioxidant synergy is considered an advantage in adding polyphenols from varied plants. This study investigated the antioxidant and synergistic interactions among olive leaf (OL), bay laurel (BL), and rosemary (RL) leaf powder mixture (LPM: OL + BL + RL), using in vitro chemical tests [TPC, ORAC, TEAC-ABTS, FRAP; combination index (CI)], and in vivo validation on blood oxidative status, metabolic profile, and intestinal histomorphology in laying hens. The in vitro study indicated a whole higher antioxidant capacity for the LPM than respective single/double-leave combinations. The LPM CI value (IC50, 0.60) indicated a synergistic effect compared to the binary combinations. Thus, the LPM was validated in vivo through dietary supplementation on sixty Lohmann Brown hens (30 weeks old), reared in an indoor-outdoor rearing system divided. The hens were allocated into two experimental groups (n. 30): basal control diet group; and diet supplemented group with 6 g/kg feed of LPM) containing OL, BL, and RL (respectively, at 65.7%:18.9%:15.4%), for 60 days. The LPM improved (
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