Small but Mighty: A Microfluidic Biofuel Cell-Based Biosensor for the Determination of Ethanol.

Jirawan Monkratok, Pattanaphong Janphuang, Kantapat Chansaenpak, Sireerat Lisnund, Vincent Blay, Piyanut Pinyou

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Biofuel Cells Based on Oxidoreductases and Electroactive Nanomaterials: Development and Characterization.Biosensors · 2025
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jirawan MonkratokSchool of Chemistry, Institute of Science, Suranaree University of Technology, 111 University Ave., Nakhon Ratchasima 30000, Thailand.

Pattanaphong JanphuangSynchrotron Light Research Institute (Public Organization), 111 University Ave., Nakhon Ratchasima 30000, Thailand.

Kantapat ChansaenpakNational Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani 12120, Thailand.

Sireerat LisnundDepartment of Applied Chemistry, Faculty of Science and Liberal Arts, Rajamangala University of Technology Isan, 744, Suranarai Rd., Nakhon Ratchasima 30000, Thailand.

Vincent BlayDepartment of Microbiology and Environmental Toxicology, University of California at Santa Cruz, Santa Cruz, CA 95064, USA.ORCID 0000-0001-9602-2375

Piyanut PinyouSchool of Chemistry, Institute of Science, Suranaree University of Technology, 111 University Ave., Nakhon Ratchasima 30000, Thailand.ORCID 0000-0001-7978-1898

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a membraneless-microfluidic biofuel cell (MBFC) for the quantification of ethanol. The system employs anolyte and catholyte solutions, each containing a biocatalyst and redox mediator. The laminar flow conditions in the microfluidic chip minimize the mixing between anolyte and catholyte and obviate the need for a membrane to separate them. When ethanol is added to the anolyte, alcohol dehydrogenase (ADH) catalyzes its oxidation to acetaldehyde, releasing electrons to the anode. On the cathode, electrons are transferred to horseradish peroxidase (HRP), which reduces hydrogen peroxide in the catholyte to water. We optimized key design factors and operating conditions. We also studied the incorporation of glycerol as a viscosity modifier, which improved the power and current density supplied by the MBFC, with a maximum power output of 307 µW cm

Indexed as

Bioelectric Energy SourcesBiofuelsBiosensing TechniquesEthanolMicrofluidicsAlcohol DehydrogenaseElectrodesHorseradish PeroxidaseHydrogen PeroxideOxidation-ReductionAlcohol DehydrogenaseBiofuelsEthanolHorseradish PeroxidaseHydrogen Peroxidealcohol dehydrogenasebiofuel cellbiosensorethanolhorseradish peroxidasemicrofluidics

Identifiers

PMID39942777

DOI10.3390/molecules30030673

PMCPMC11821114

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.