Monitoring Dynamic Changes of Cellular Membrane GSH During Stroke via an ESIPT-Based Near-Infrared Fluorescent Probe.

Yue Gao, Zhao Wang

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

2 authors.

Yue GaoHubei Provincial Engineering Research Center of Racing Horse Detection and Application Transformation, Equine Science Research and Horse Doping Control Laboratory, College of Food Science and Technology, Wuhan Business University, Wuhan 430056, China.

Zhao WangHubei Provincial Engineering Research Center of Racing Horse Detection and Application Transformation, Equine Science Research and Horse Doping Control Laboratory, College of Food Science and Technology, Wuhan Business University, Wuhan 430056, China.

Funding

Hubei Provincial National Natural Science Foundation ,Scientific Research Program of Hubei Provincial Department of Education,Wuhan Business University Doctoral Fund Project 2022CFB660,B2022288,2023KB011

6 · The paper itself

Abstract

Stroke, primarily ischemic (85%), results from inadequate blood supply and is worsened by ferroptosis, characterized by free radical generation and lipid peroxidation. Monitoring ferroptosis is essential for understanding its mechanisms and developing treatments. Glutathione (GSH) is a key ferroptosis biomarker, but current probes are limited by short excitation/emission wavelengths, small Stokes shifts, and inability to monitor dynamic GSH changes at the cellular membrane, where ferroptosis plays a crucial role. To address these issues, we developed the PM-Red-GSH, a novel near-infrared (NIR) probe based on the Excited-state intramolecular proton transfer (ESIPT) mechanism. It shows strong NIR emission (715 nm), large Stokes shift (290 nm), and enhanced membrane binding (PCC = 0.95) due to its alkyl group. PM-Red-GSH enables dynamic GSH monitoring in an MCAO mouse model. These findings offer new insights into ferroptosis and stroke treatment.

Indexed as

Cell MembraneFluorescent DyesGlutathioneStrokeAnimalsDisease Models, AnimalFerroptosisHumansLipid PeroxidationMiceProtonsFluorescent DyesGlutathioneProtonsexcited-state intramolecular proton transferferroptosisglutathionenear-infrared probestroke

Identifiers

PMID39942696

DOI10.3390/molecules30030592

PMCPMC11820386

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.