Evidence map›Paper›PMID 39940864›Full record

ArticleInternational journal of molecular sciences2025

Multi-Omics Sequencing Dissects the Atlas of Seminal Plasma Exosomes from Semen Containing Low or High Rates of Sperm with Cytoplasmic Droplets.

Zilu Zhang, Xiaoxian Xu, Fumei Chen, Qingyou Liu, Zhili Li, Xibang Zheng, Yunxiang Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zilu ZhangCollege of Animal Science & Technology, Guangxi University, Nanning 530004, China.
Xiaoxian XuCollege of Animal Science & Technology, Guangxi University, Nanning 530004, China.
Fumei ChenCollege of Animal Science & Technology, Guangxi University, Nanning 530004, China.
Qingyou LiuCollege of Animal Science and Technology, Foshan University, Foshan 528231, China.ORCID 0000-0003-3265-540X
Zhili LiCollege of Animal Science and Technology, Foshan University, Foshan 528231, China.
Xibang ZhengCollege of Animal Science & Technology, Guangxi University, Nanning 530004, China.
Yunxiang ZhaoCollege of Animal Science & Technology, Guangxi University, Nanning 530004, China.ORCID 0009-0005-9620-7640

Funding

Guangxi Science and Technology Department KY01030033424006
6 · The paper itself

Abstract

Sperm cytoplasmic droplets (CDs) are remnants of cytoplasm that can cause a number of problems if it not shed from the sperm after ejaculation. Exosomes can rapidly bind to sperm, but it is not clear whether exosomes can affect the migration and shedding of CDs. We first extracted and characterized seminal plasma exosomes from boar semen containing sperm with low or high rates of CDs. Then, the transcriptomic and proteomic detection of these exosomes were performed to analyze the differences between the two groups of seminal plasma exosomes. The results revealed that 486 differentially expressed genes (DEGs), 40 differentially expressed proteins (DEPs), and 503 differentially expressed lncRNAs (DElncRNAs) were identified between the low CD rate group and high CD rate group. Integrative multi-omics analysis showed that exosome components may affect migration and shedding of cytoplasmic droplets by influencing cytoskeletal regulation and insulin signaling, including regulation of the actin cytoskeleton, ECM-receptor interaction, axon guidance, insulin secretion, and the insulin signaling pathway. Overall, our study systematically revealed the DEGs, DEPs, and DElncRNAs in seminal plasma exosomes between low CD rate semen and high CD rate semen, which will help broaden our understanding of the complex molecular mechanisms involved in the shedding of CDs.

Indexed as

CytoplasmExosomesSemenSpermatozoaAnimalsGene Expression ProfilingMaleMultiomicsProteomicsRNA, Long NoncodingSwineTranscriptomeRNA, Long Noncodingcytoplasmic dropletsexosomesproteomicstranscriptomics

Identifiers

PMID39940864
PMCPMC11817786

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.