Evidence map›Paper›PMID 39940725›Full record

ArticleInternational journal of molecular sciences2025

Development of Sheep Intestinal Organoids for Studying Deoxynivalenol-Induced Toxicity.

Hongyu Wang, Xige He, Miaomiao Zhang, Na Fan, Zongxuan Yang, Ting Shen, Jiaojiao Guo, Yongli Song, Guifang Cao, Yongbin Liu and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hongyu WangInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.ORCID 0009-0005-6799-3128
Xige HeInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.
Miaomiao ZhangInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.
Na FanInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.
Zongxuan YangInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.
Ting ShenInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.
Jiaojiao GuoInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.
Yongli SongInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.
Guifang CaoInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.
Yongbin LiuInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.
Xihe LiInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.ORCID 0000-0002-7001-7997
Buhe NashunInner Mongolia Key Laboratory for Molecular Regulation of the Cell, Inner Mongolia University, Hohhot 010070, China.

Funding

'Leading the Charge with Open Competition' project of Inner Mongolia 2022JBGS0021the Fund for Excellent Young Scholars of Inner Mongolia 2021JQ04the National Key Research and Development Program of China 2022YFA0806200the National Natural Science Foundation of China 31760335the National Natural Science Foundation of China 31970759the National Natural Science Foundation of China 32160145
6 · The paper itself

Abstract

Sheep are an important livestock species whose gastrointestinal tract is essential for overall health. Feed contaminants such as bacterial toxins and mycotoxins severely damage the sheep intestine, yet the mechanisms remain mostly elusive partially due to the lack of physiologically relevant in vitro models. Here, we investigated molecular mechanisms underlying deoxynivalenol (DON)-induced toxicity by developing intestinal organoids from isolated intestinal crypts of Hu sheep. The organoids had a central lumen and monolayer epithelium, and could be continuously passaged, cryopreserved, and resuscitated. Histological and transcriptomic analysis showed that the intestinal organoids recapitulate the cell lineages and gene expression characteristics of the original intestinal tissues. Statistical analysis indicated that DON exposure significantly inhibited organoid formation efficiency, as well as the proliferation and activity of intestinal organoid cells. RNA-seq and Western blotting analysis further revealed that DON exposure induces intestinal toxicity by inhibiting the PI3K/AKT/GSK3β/β-catenin signaling pathway. Our study provides a novel example of organoid application in toxicity studies and reveals the signaling pathway involved in DON-induced toxicity in sheep, which is of great significance for improving mitigation strategies for DON.

Indexed as

IntestinesOrganoidsTrichothecenesAnimalsCell ProliferationGlycogen Synthase Kinase 3 betaIntestinal MucosaPhosphatidylinositol 3-KinasesSheepSignal TransductiondeoxynivalenolGlycogen Synthase Kinase 3 betaPhosphatidylinositol 3-KinasesTrichothecenesdeoxynivalenol (DON)intestinal organoidsintestinal toxicityPI3K/AKT/GSK3β/β-catenin signalingsheep

Identifiers

PMID39940725
PMCPMC11816529

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.