Evidence map›Paper›PMID 39940125›Full record

ArticleNanomaterials (Basel, Switzerland)2025

A Novel Nanomaterial-Based Approach for the Cryopreservation of Individual Sperm Cells Using Addressable Nanoliter Containers.

Bat-Sheva Galmidi, Yana Shafran, Chen Shimon, Adva Aizer, Raoul Orvieto, Naomi Zurgil, Mordechai Deutsch, Zeev Schiffer, Dror Fixler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanomaterials (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Bat-Sheva GalmidiThe Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Yana ShafranThe Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Chen ShimonDepartment of Gynecology and Fertility, Sheba Medical Center, Tel HaShomer, Ramat-Gan 5262000, Israel.
Adva AizerDepartment of Gynecology and Fertility, Sheba Medical Center, Tel HaShomer, Ramat-Gan 5262000, Israel.ORCID 0000-0003-0820-8079
Raoul OrvietoDepartment of Gynecology and Fertility, Sheba Medical Center, Tel HaShomer, Ramat-Gan 5262000, Israel.ORCID 0000-0002-0865-1196
Naomi ZurgilThe Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Mordechai DeutschThe Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and Technology of the Cellome, Physics Department, Bar-Ilan University, Ramat-Gan 5290002, Israel.
Zeev SchifferDepartment of Laser Physics, Elbit Systems, Rehovot 7670305, Israel.
Dror FixlerFaculty of Engineering, The Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 5290002, Israel.ORCID 0000-0003-0963-7908

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The research and development of a matrix of Addressable Nanoliter Containers (ANLCs) is the focus of this work. ANLCs introduce a novel approach for cryopreserving single sperm cells. A significant increase in sperm cell mortality was observed after cryopreserving nanoliter-scale cell suspensions, attributed to the diffusion of water from the aqueous droplets into the surrounding oil phase. This process elevated the salt concentration within the droplets. A practical solution was devised by saturating the oil with water, significantly reducing the concentration gradient and, consequently, the diffusion. For ANLCs smaller than a few nanoliters, locating individual sperm cells within the containers became highly feasible. Using saturated oil, the survival rate reached 100%. Optical simulations were conducted to evaluate the impact of ANLCs on light scattering, enabling the selection of designs with minimal scattering. The simulations conclusively demonstrated that a cylindrical container with a flat bottom produced the least light scattering. This device was tested under clinical conditions in an in vitro fertilization (IVF) laboratory, revealing its strong potential as a practical tool for housing individual sperm cells. It enables characterization using interferometric indicators and facilitates the selection of sperm cells for IVF.

Indexed as

cryopreservationdiffusionIVFnanolitersperm cells

Identifiers

PMID39940125
PMCPMC11821078

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.