Evidence map›Paper›PMID 39939952›Full record

ArticleBMC complementary medicine and therapies2025

Ribes nigrum leaf extract downregulates pro-inflammatory gene expression and regulates redox balance in microglial cells.

Alvard Minasyan, Vivien Pires, Catherine Gondcaille, Mikayel Ginovyan, Marika Mróz, Stéphane Savary, Mustapha Cherkaoui-Malki, Barbara Kusznierewicz, Agnieszka Bartoszek, Pierre Andreoletti and 1 more

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Article in BMC complementary medicine and therapies, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Frontiers in nutrition · 2026
    Review
  2. The Current State of Knowledge onPlants (Basel, Switzerland) · 2025
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Alvard MinasyanResearch Institute of Biology, Department of Biochemistry, Microbiology & Biotechnology, Yerevan State University, 1 A. Manoogian Str., Yerevan, 0025, Armenia.
Vivien PiresCentre des Sciences du Goût et de l'Alimentation, Institut Agro, CNRS, INRAE, Université de Bourgogne, Dijon, F-21000, France.
Catherine GondcailleCentre des Sciences du Goût et de l'Alimentation, Institut Agro, CNRS, INRAE, Université de Bourgogne, Dijon, F-21000, France.
Mikayel GinovyanResearch Institute of Biology, Department of Biochemistry, Microbiology & Biotechnology, Yerevan State University, 1 A. Manoogian Str., Yerevan, 0025, Armenia.
Marika MrózDepartment of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, Gdańsk, 80-233, Poland.
Stéphane SavaryCentre des Sciences du Goût et de l'Alimentation, Institut Agro, CNRS, INRAE, Université de Bourgogne, Dijon, F-21000, France.
Mustapha Cherkaoui-MalkiCentre des Sciences du Goût et de l'Alimentation, Institut Agro, CNRS, INRAE, Université de Bourgogne, Dijon, F-21000, France.
Barbara KusznierewiczDepartment of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, Gdańsk, 80-233, Poland.
Agnieszka BartoszekDepartment of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12, Gdańsk, 80-233, Poland.
Pierre AndreolettiCentre des Sciences du Goût et de l'Alimentation, Institut Agro, CNRS, INRAE, Université de Bourgogne, Dijon, F-21000, France.
Naira SahakyanResearch Institute of Biology, Department of Biochemistry, Microbiology & Biotechnology, Yerevan State University, 1 A. Manoogian Str., Yerevan, 0025, Armenia. sahakyannaira@ysu.am.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThis study focuses on the investigation of the antioxidant and anti-inflammatory activities of alcohol extracts from Ribes nigrum leaves on murine BV-2 microglial Wt and Acyl-CoA oxidase 1 deficient (Acox1

methodsThe extract chemical composition was analyzed via LC-Q-Orbitrap HRMS. Various assays, including DPPH, MTT, and H

resultsInvestigations showed the presence of phenolic compounds in this extract, among which flavan-3-ols, flavonols, furanocoumarins, hydroxycinnamates were major components, which are known for their biological activity in various test systems. The MTT test revealed a concentration of 0.125 mg/mL of R. nigrum extract as the highest non-toxic. The investigated extract showed high antioxidant activity in chemical-based tests. The antioxidant potential of the R. nigrum leaf extract was furtherly explored using the BV-2 microglial cell line models. Moreover, the extract was found to alter the activity of the main antioxidant enzyme, catalase and fatty acid oxidation enzyme, Acyl-CoA oxidase 1 (ACOX1) as well as the expression of appropriate genes in Wt and Acox1

conclusionOur study demonstrated the protective effects of R. nigrum leaf extracts on BV-2 microglial cells by reducing oxidative and nitrosative stress, decreasing pro-inflammatory gene expression, and normalizing peroxisomal function, highlighting the potential of these extracts as therapeutic agents for managing oxidative stress and inflammation.

Indexed as

Anti-Inflammatory AgentsAntioxidantsGene ExpressionMicrogliaPlant ExtractsAnimalsCell LineDown-RegulationMiceOxidation-ReductionPlant LeavesReactive Oxygen SpeciesAnti-Inflammatory AgentsAntioxidantsPlant ExtractsReactive Oxygen SpeciesArmenian floraNeurodegenerative disordersOxidative stressPolyphenolsReactive oxygen species

Identifiers

PMID39939952
PMCPMC11823126

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.