ArticleNature communications2025
Dense, continuous membrane labeling and expansion microscopy visualization of ultrastructure in tissues.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- High-Resolution Detection of Sphingolipids During Chlamydia trachomatis Infection via Expansion Microscopy.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Sphingolipids associate with the chlamydial nucleoid and mark developmental transitions in Chlamydia trachomatis.Nature communications · 2026Article
- Hydrogel Chemistry in Expansion Microscopy: From Polyacrylamide to Next-Generation Swellable Polymers.Chembiochem : a European journal of chemical biology · 2026Review
- Review
- HAK-actin, a U-ExM-compatible probe to image the actin cytoskeleton.Cell reports methods · 2026Article
- Landscape expansion microscopy reveals interactions between membrane and phase-separated organelles.The Journal of cell biology · 2026Article
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Expansion omics: from expansion microscopy to spatial omics.Molecular systems biology · 2026Review
- Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy.Npj viruses · 2026Review
- Landscape Expansion Microscopy Reveals Interactions between Membrane and Phase-Separated Organelles.bioRxiv : the preprint server for biology · 2025Article
- Connectomics beyond electron microscopy.Nature methods · 2025Article
- All-optical visualization of specific molecules in the ultrastructural context of brain tissue.Nature biotechnology · 2025Article
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Comparative prospects of imaging methods for whole-brain mammalian connectomics.Cell reports methods · 2025Review
- Data-driven synapse classification reveals a logic of glutamate receptor diversity.bioRxiv : the preprint server for biology · 2025Article
- Expansion microscopy reveals neural circuit organization in genetic animal models.Neurophotonics · 2025Review
- Single-shot 20-fold expansion microscopy.Nature methods · 2024Article
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19 authors.
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Abstract
Lipid membranes are key to the nanoscale compartmentalization of biological systems, but fluorescent visualization of them in intact tissues, with nanoscale precision, is challenging to do with high labeling density. Here, we report ultrastructural membrane expansion microscopy (umExM), which combines an innovative membrane label and optimized expansion microscopy protocol, to support dense labeling of membranes in tissues for nanoscale visualization. We validate the high signal-to-background ratio, and uniformity and continuity, of umExM membrane labeling in brain slices, which supports the imaging of membranes and proteins at a resolution of ~60 nm on a confocal microscope. We demonstrate the utility of umExM for the segmentation and tracing of neuronal processes, such as axons, in mouse brain tissue. Combining umExM with optical fluctuation imaging, or iterating the expansion process, yields ~35 nm resolution imaging, pointing towards the potential for electron microscopy resolution visualization of brain membranes on ordinary light microscopes.
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