Evidence map›Paper›PMID 39938190›Full record

ArticleExperimental and molecular pathology2025

Hyperinsulinemic male LEW.1WR1 rats show early signs of impaired liver metabolism.

Madushika Wimalarathne, Bailey L Bowser, Albert B Arul, Quiana C Wilkerson-Vidal, Moses A David, Emily C Hunt, Helen Gibson, Renã A S Robinson, Sharifa T Love-Rutledge

Abstract read
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In one paragraph

Article in Experimental and molecular pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Madushika WimalarathneDepartment of Chemistry, The University of Alabama in Huntsville, Huntsville, AL 35899, United States of America.
Bailey L BowserDepartment of Chemistry, Vanderbilt University, Nashville, TN 37235, United States of America.
Albert B ArulDepartment of Chemistry, Vanderbilt University, Nashville, TN 37235, United States of America.
Quiana C Wilkerson-VidalDepartment of Chemistry, The University of Alabama in Huntsville, Huntsville, AL 35899, United States of America.
Moses A DavidDepartment of Chemistry, The University of Alabama in Huntsville, Huntsville, AL 35899, United States of America.
Emily C HuntDepartment of Chemistry, The University of Alabama in Huntsville, Huntsville, AL 35899, United States of America.
Helen GibsonDepartment of Biology, The University of Alabama in Huntsville, Huntsville, AL 35899, United States of America.
Renã A S RobinsonDepartment of Chemistry, Vanderbilt University, Nashville, TN 37235, United States of America.
Sharifa T Love-RutledgeDepartment of Chemistry, The University of Alabama in Huntsville, Huntsville, AL 35899, United States of America. Electronic address: sharifa.love-rutledge@uah.edu.

Funding

Novel Multiplexing Proteomics to Study the Periphery in Alzheimer's DiseaseR01GM117191 · NIGMS · VANDERBILT UNIVERSITY · PI ROBINSON, RENA A. S. · 2016 to 2020
$2.1M
NIGMS NIH HHS R01 GM117191
6 · The paper itself

Abstract

Weanling LEW.1WR1 (1WR1) rats are susceptible to type 1 diabetes (T1D) and hyperinsulinemic. Similar to human patients with T1D, these animals are susceptible to developing fatty liver infiltrates. Insulin resistance-related steatosis can lead to the development of severe forms of nonalcoholic fatty liver disease (NAFLD). Previous work in 1WR1 rats suggests that in the absence of T1D, they have increased body mass that is not reconciled by measuring their abdominal fat pads; this suggests 1WR1 rats have an underlying predisposition to store fat in the liver unrelated to diabetes status. We hypothesized that 1WR1 rats show early signs of NAFLD development. We assessed proteomics changes in the livers of glucose intolerant and hyperinsulinemic young adult 1WR1 rats to identify early detectable characteristics of NAFLD development. Our results show young adult 1WR1 rats have UBD/FAT10 gene over expression in the liver. Additionally, they have decreased mitochondrial protein levels, which may lead to lipid accumulation in the liver. A quantitative proteomic analysis showed protein expression related to branch chain fatty acid metabolism, fatty acid beta-oxidation, and oxidative phosphorylation in the liver is significantly different in 1WR1 rats compared to control LEW/SsNHsd (SsNHsd) rats. In summary, our study shows that 1WR1 rats developed early characteristics of mitochondrial dysfunction and insulin resistance in the liver independent of T1D, which are commonly observed with NAFLD development.

Indexed as

Fatty LiverHyperinsulinismLiverAnimalsDiabetes Mellitus, Type 1Insulin ResistanceLipid MetabolismMaleNon-alcoholic Fatty Liver DiseaseOxidative PhosphorylationProteomicsRatscPILOT multiplexLEW.1WR1 ratLipid metabolismMetabolic dysfunction associated steatotic liver diseaseMitochondrial dysfunctionNonalcoholic fatty liver diseaseProteomics

Identifiers

PMID39938190
PMCPMC12061046

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.