Evidence map›Paper›PMID 39931768›Full record

ArticleZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences

Reprogramming astrocytes into dopaminergic neurons to restore motor dysfunction in Parkinson

Chunbo Liu, Mengjiao Ying, Ao Wang, Yumeng Liu, Ying Chen, Wenhao Ye, Hebao Wen, Caiyun Ma, Changqing Liu, Yu Guo

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Article in Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Chunbo LiuSchool of Life Sciences, Bengbu Medical University, Bengbu Anhui 233030. lcb1473493910@163.com.
Mengjiao YingSchool of Life Sciences, Bengbu Medical University, Bengbu Anhui 233030.
Ao WangSchool of Laboratory Medicine, Bengbu Medical University, Bengbu Anhui 233030.
Yumeng LiuSchool of Life Sciences, Bengbu Medical University, Bengbu Anhui 233030.
Ying ChenSchool of Life Sciences, Bengbu Medical University, Bengbu Anhui 233030.
Wenhao YeSchool of Life Sciences, Bengbu Medical University, Bengbu Anhui 233030.
Hebao WenDepartment of Sports and Art, Bengbu Medical University, Bengbu Anhui 233030, China.
Caiyun MaSchool of Life Sciences, Bengbu Medical University, Bengbu Anhui 233030.
Changqing LiuSchool of Life Sciences, Bengbu Medical University, Bengbu Anhui 233030.
Yu GuoSchool of Laboratory Medicine, Bengbu Medical University, Bengbu Anhui 233030. guoyu@bbmu.edu.cn.

Funding

the Anhui University Student Innovation Training Program S202210367043the Key Project of Natural Science Foundation of Universities of Anhui Province KJ2021ZD0085, KJ2021A0784, 2022AH051434 and 2024AH051296the Key Research and Development Program of Anhui Province 2022e07020030 and 2022e07020032the Major Science and Technology Project Plan of Bengbu Medical College 2021byfy002 and 2023byfy007the National Innovative Training Program for College Students 202210367019, 202310367005 and 202310367061
6 · The paper itself

Abstract

objectivesParkinson's disease (PD) is a neurodegenerative disorder primarily caused by the loss of dopaminergic neurons (DA) in the brain. Since DA neurons are non-renewable, conventional therapies only alleviate symptoms without addressing the root cause. This study aims to reprogram astrocyte (AS) into DA neurons for transplantation into the brain to reconstruct damaged neural circuits and treat PD.

methodsAstrocytes were isolated from neonatal rat brain tissues. A lentiviral vector carrying the transcription factors nuclear receptor-related factor 1 (UNRR1) and achaete-scute family bHLH transcription factor 1 (ASCL1), named LV-NURR1-ASCL1, was constructed and used to infect cultured rat AS in vitro. Immunofluorescence, Western blotting, and reverse transcription polymerase chain reaction (RT-PCR) were employed to detect and compare the expression levels of

resultsImmunofluorescence analysis showed positive expression of NURR1 and ASCL1 in AS after lentiviral infection. RT-PCR results demonstrated that the mRNA expression levels of

conclusionsLentiviral overexpression of

Indexed as

AstrocytesBasic Helix-Loop-Helix ProteinsDisease Models, AnimalDopaminergic NeuronsLentivirusNuclear Receptor Subfamily 4, Group A, Member 2Parkinson DiseaseAnimalsCells, CulturedCellular ReprogrammingGenetic VectorsHepatocyte Nuclear Factor 3-betaMaleRatsRats, Sprague-DawleyAscl1 protein, ratBasic Helix-Loop-Helix ProteinsFoxa2 protein, ratHepatocyte Nuclear Factor 3-betaNr4a2 protein, ratNuclear Receptor Subfamily 4, Group A, Member 2astrocytescell transdifferentiationdopaminergic neuronsparkinson’s diseasetranscription factors

Identifiers

PMID39931768
PMCPMC11814384

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.