Electrically Driven, Bioluminescent Compliant Devices for Soft Robotics.

Soft robotics, a research field wherein robots are fabricated from compliant materials, has sparked widespread research interest because of its potential applications in a variety of scenarios. In soft robots, luminescence is an important functionality for communication and information transmission, and it is typically achieved through electroluminescence, which relies on synthetic substances activated by external electric sources, such as batteries. This paper focuses on the use of luciferase, a biologically derived luminescent enzyme, as a luminescent material. Bioluminescence, which is triggered by the luciferin-luciferase reaction, is highly energy-efficient, nontoxic, and eco-friendly. In this regard, a mammalian cell-derived secreted luciferase bioluminescent liquid was developed. This bioluminescent liquid is strongly bright, stable, freezable, and scalable for use as a soft robotic material. To investigate the applicability of this bioluminescent liquid to soft robotics, it was incorporated as an electrode in electrically driven soft actuators, sensors, and robots. Specifically, dielectric elastomer sensors (DESs) and dielectric elastomer actuators (DEAs) were fabricated and characterized using established fabrication processes. The resistivity of the bioluminescent liquid was found to be 448.1 Ω·cm. When the DES was subjected to uniaxial strain, it exhibited a linear response and large deformation of up to 200% strain, with a simultaneous luminance change of 27%. The DEA displayed an areal strain of 46.0% and a luminance change of 31% at an applied voltage of 3.4 kV. The waterproof bending DEA generated a tip angle of 21.8° at 10 kV and was applied to a jellyfish robot that could swim in water at a speed of 2.1 mm/s. The experimental results demonstrated the successful operation of these devices, validating the concept of energy-efficient, safe, and environmentally friendly bioluminescent soft robots.