ArticleGenome biology2025
Long-read sequencing and genome assembly of natural history collection samples and challenging specimens.
Article in Genome biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Complete mitochondrial genome characterization and exploratory mitochondrial DNA modification signals in square-head catfish Chaca chaca.Scientific reports · 2026Article
- From Permits to Samples: Addressing Key Challenges for High-Quality Reference Genome Generation in Europe.Molecular ecology resources · 2026Review
- The effect of human-specific genetic variants on neuronal spinogenesis.Frontiers in genetics · 2026Review
- CiFi: accurate long-read chromosome conformation capture with low-input requirements.Nature communications · 2025Article
- Peptide Mass Fingerprinting of South American Xenarthrans: A New Resource for Zooarcheology and Palaeontology.Journal of proteome research · 2025Article
- Unlocking natural history collections to improve eDNA reference databases and biodiversity monitoring.Bioscience · 2025Article
- Genomic Signatures in Maned Three-Toed Sloths From Ancient to Recent Environmental Changes in Brazil's Threatened Atlantic Forest.Molecular ecology · 2025Article
- Draft genome of the Cuban Painted Landsnail Polymita picta, International Mollusc of the year 2022.BMC genomic data · 2025Article
- Establishing genome sequencing and assembly for non-model and emerging model organisms: a brief guide.Frontiers in zoology · 2025Review
- Improving taxonomic inference from ancient environmental metagenomes by masking microbial-like regions in reference genomes.GigaScience · 2025Article
- Chromosome-level genome assembly of the sacoglossan sea slug Elysia timida (Risso, 1818).BMC genomics · 2024Article
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20 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protocol, employing an alternative polymerase to overcome PCR bias, we assemble the 3.1 Gb maned sloth genome, surpassing the previous 500 Mb protocol size limit. Our protocol also improves assemblies of other difficult-to-sequence molluscs and arthropods, including millimeter-sized organisms. By highlighting collections as valuable sample resources and facilitating genome assembly of tiny and challenging organisms, our study advances efforts to obtain reference genomes of all eukaryotes.
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