Evidence map›Paper›PMID 39930313›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Generation of Retinal Organoids Using Human-Induced Pluripotent Stem Cells.

Keith Theodore, Joel Alan Imventarza, Saleha Tahir, Bruna Lopes da Costa, Peter M J Quinn

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Keith TheodoreFM Kirby Center for Molecular Ophthalmology, Department of Ophthalmology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Joel Alan ImventarzaDepartment of Ophthalmology, Columbia University Irving Medical Center, New York, NY, USA.
Saleha TahirFM Kirby Center for Molecular Ophthalmology, Department of Ophthalmology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Bruna Lopes da CostaDepartment of Ophthalmology, Columbia University Irving Medical Center, New York, NY, USA. bl2755@cumc.columbia.edu.
Peter M J QuinnFM Kirby Center for Molecular Ophthalmology, Department of Ophthalmology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. peter.quinn1@pennmedicine.upenn.edu.

Funding

Prime editing for Crumbs homologue 1 (CRB1) Inherited Retinal DystrophiesR01EY034952 · NEI · UNIVERSITY OF PENNSYLVANIA · PI Peter Martin John Quinn · 2023 to 2026
$1.6M
NEI NIH HHS R01 EY034952
6 · The paper itself

Abstract

Retinal organoids (ROs) derived from human-induced pluripotent stem cells (hiPSCs) serve as relevant models for studying retinal disease pathogenesis, as well as furthering gene therapy efforts. These complex, three-dimensional (3D), multicellular structures recapitulate the development and functionality of the maturing human retina. Here, we describe an in-depth method for the generation of ROs from hiPSCs and evaluate the morphology of these multilayered structures.

Indexed as

Cell Culture TechniquesInduced Pluripotent Stem CellsOrganoidsRetinaCell DifferentiationHumansHuman-induced pluripotent stem cells (hiPSCs)Retinal organoids

Identifiers

PMID39930313

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.