Evidence map›Paper›PMID 39930222›Full record

ArticleAdvances in experimental medicine and biology2025

IGFBP5 as a Novel Basolateral Secretion Marker in the Retinal Pigment Epithelium.

Kaitlyn M Digsby, Qitao Zhang, Nirosha D Perera, Anthanasios Karoukis, Aaron DenDekker, Abigail T Fahim, Jason Matthew Lewis Miller

Abstract read
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In one paragraph

Article in Advances in experimental medicine and biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kaitlyn M DigsbyDepartment of Pharmacology, University of Michigan, Ann Arbor, MI, USA.
Qitao ZhangDepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, USA.
Nirosha D PereraDepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, USA.
Anthanasios KaroukisDepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, USA.
Aaron DenDekkerDepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, USA.
Abigail T FahimDepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, USA.
Jason Matthew Lewis MillerDepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, USA. miljason@umich.edu.

Funding

Interdepartmental Training in Pharmacological SciencesT32GM140223 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Lori L Isom · 2021 to 2026
$3.8M
Lipid Trafficking and Metabolism in the Retinal Pigment EpitheliumK08EY033420 · NEI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Jason Matthew-Lewis Miller · 2022 to 2026
$1.2M
NEI NIH HHS K08 EY033420NIGMS NIH HHS T32 GM140223
6 · The paper itself

Abstract

The retinal pigment epithelium (RPE) is a highly polarized cell type in the retina which is specialized to support the photoreceptors and choroid. To assess the adequacy of RPE polarity in culture or altered RPE polarity under disease conditions in vivo, the polarized secretion of particular proteins can be monitored. Currently accepted basolateral secretion markers for RPE include the pro-angiogenic factor, VEGF, and extracellular matrix remodeling protein, TIMP-3. However, VEGF secretion is low enough that it often requires ELISA for detection, and TIMP-3 secretion is only modestly polarized to the basolateral side. We sought out an improved marker of basolateral secretion that is both highly expressed and highly polarized. Through a combination of bioinformatics analysis and RPE cell culture experiments, we identify insulin growth factor binding protein 5 (IGFBP5) as a more robust and convenient RPE basolateral marker compared to TIMP-3 and VEGF.

Indexed as

Insulin-Like Growth Factor Binding Protein 5Retinal Pigment EpitheliumAnimalsBiomarkersCell PolarityHumansTissue Inhibitor of Metalloproteinase-3Vascular Endothelial Growth Factor ABiomarkersInsulin-Like Growth Factor Binding Protein 5TIMP3 protein, humanTissue Inhibitor of Metalloproteinase-3Vascular Endothelial Growth Factor ABasolateral secretionIGFBP5Retinal pigment epitheliumSecreted polarity marker

Identifiers

PMID39930222
PMCPMC12254955

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.