Review in Nature protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what money
Authors and funding
11 authors.
Kevin C Gonzalez *Department of Neuroscience, Columbia University, New York, NY, USA.
Asako Noguchi *Department of Neuroscience, Columbia University, New York, NY, USA.
George ZakkaDepartment of Neuroscience, Columbia University, New York, NY, USA.
Hyun Choong YongDepartment of Neuroscience, Columbia University, New York, NY, USA.ORCID 0000-0002-1841-6317
Satoshi TeradaDepartment of Neuroscience, Columbia University, New York, NY, USA.
Miklos SzoboszlayDepartment of Neuroscience, Columbia University, New York, NY, USA.ORCID 0000-0001-5736-2967
Justin O'HareDepartment of Neuroscience, Columbia University, New York, NY, USA.
Adrian NegreanDepartment of Neuroscience, Columbia University, New York, NY, USA.ORCID 0000-0002-9256-7412
Tristan GeillerDepartment of Neuroscience, Columbia University, New York, NY, USA.
Franck PolleuxDepartment of Neuroscience, Columbia University, New York, NY, USA.
Attila LosonczyDepartment of Neuroscience, Columbia University, New York, NY, USA. al2856@columbia.edu.
Funding
Development, Maintenance, and Human-Specific Evolution of Cortical CircuitsR35NS127232 · NINDS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI FRANCK POLLEUX · 2022 to 2026
$6.1M
Optimization, application and dissemination of high-speed hybrid multiphoton volumetric imaging technologiesU01NS115530 · NINDS · ROCKEFELLER UNIVERSITY · PI LOSONCZY, ATTILA, VAZIRI, ALIPASHA · 2020 to 2023
$4.7M
Microcircuit, cellular and molecular dissection of impaired hippocampal function in a mouse model of the 22q11.2 deletionR01MH124047 · NIMH · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI GOGOS, JOSEPH A, LOSONCZY, ATTILA · 2020 to 2024
$3.8M
Experimental and modeling investigations into microcircuit, cellular and subcellular determinants of hippocampal ensemble recruitment to contextual representationsR01MH124867 · NIMH · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI LOSONCZY, ATTILA, POIRAZI, PANAYIOTA · 2021 to 2025
$3.4M
Control of Axon Initial Segment in EpilepsyR01NS121106 · NINDS · STANFORD UNIVERSITY · PI LOSONCZY, ATTILA, SOLTESZ, IVAN · 2021 to 2025
$3.4M
ADVANCED GRADUATE TRAINING PROGRAM IN NEUROBIOLOGY & BEHAVIORT32NS064928 · NINDS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI HENGST, ULRICH · 2010 to 2024
$3.0M
Local Circuit Control of Rapid Plasticity and Tunable Ensemble Formation in the HippocampusRF1NS133381 · NINDS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI LOSONCZY, ATTILA, SOLTESZ, IVAN · 2023 to 2023
$2.6M
Dissecting the role of the dentate gyrus microcircuit to improve cognitive discrimination in aging and Alzheimer's DiseaseRF1AG080818 · NIA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI FUSI, STEFANO, HEN, RENE · 2023 to 2023
$2.5M
Activity-dependent endocannabinoid control in epilepsyR01NS131728 · NINDS · STANFORD UNIVERSITY · PI Attila Losonczy, IVAN SOLTESZ · 2023 to 2026
$2.3M
Local Circuit Control of Rapid Plasticity and Tunable Ensemble Formation in the HippocampusR01NS133381 · NINDS · UT SOUTHWESTERN MEDICAL CENTER · PI Attila Losonczy, IVAN SOLTESZ · 2026 to 2026
$752k
Defining the Circuit, Synaptic, and Molecular Mechanisms Linking Intracellular Ca2+ Release to Learning Using Subcellularly-Targeted Manipulations and Imaging Techniques in Dendrites in VivoR00NS127815 · NINDS · UNIVERSITY OF COLORADO DENVER · PI Justin O'Hare · 2024 to 2026
$672k
Defining the Circuit, Synaptic, and Molecular Mechanisms Linking Intracellular Ca2+ Release to Learning Using Subcellularly-Targeted Manipulations and Imaging Techniques in Dendrites in VivoK99NS127815 · NINDS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI O'HARE, JUSTIN · 2022 to 2023
$272k
NIA NIH HHS RF1 AG080818NIMH NIH HHS F32 MH118716NIMH NIH HHS R01 MH124047NIMH NIH HHS R01 MH124867NINDS NIH HHS F99 NS139551NINDS NIH HHS K99 NS127815NINDS NIH HHS R00 NS127815NINDS NIH HHS R01 NS121106NINDS NIH HHS R01 NS131728NINDS NIH HHS R35 NS127232NINDS NIH HHS RF1 NS133381NINDS NIH HHS T32 NS064928NINDS NIH HHS U01 NS115530U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) R01MH124047U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) R01MH124867U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) F32MH118716U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) F99NS139551U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) K99NS127815U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) R01NS121106U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) R01NS131728U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) R01NS133381U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) R35NS127232U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS) U01NS115530U.S. Department of Health & Human Services | NIH | National Institute on Aging (U.S. National Institute on Aging) RF1AG080818
6 · The paper itself
Abstract
Sparse, single-cell labeling approaches enable high-resolution, high signal-to-noise recordings from subcellular compartments and intracellular organelles and allow precise manipulations of individual cells and local circuits while minimizing complex changes associated with global network manipulations. However, thus far, only a limited number of approaches have been developed to label single cells with unique combinations of genetically encoded indicators, target deep cortical structures or sustainably use the same chronic preparation for weeks. Here we developed a method to deliver plasmids selectively to single pyramidal neurons in the mouse dorsal hippocampus using two-photon visually guided in vivo single-cell electroporation to address these limitations. This method allows long-term plasmid expression in a controlled number of individual pyramidal neurons, facilitating subcellular resolution imaging, intracellular organelle tracking, monosynaptic input mapping, plasticity induction and targeted whole-cell patch-clamp recordings.
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Visually guided in vivo single-cell electroporation for monitoring and manipulating mammalian hippocampal neurons. · full record | OpenQuestion